Fig. 9

Four hydrophilic amino acids on the SpATA surface blocking the binding of galactomannan oligosaccharide to the predicted polysaccharide binding cleft. The polysaccharide binding cleft is indicated by the yellow shade on the enzyme surface. Amino acids in green and cyan are from different polypeptide chains. The SpATA structure was obtained from the RCSB PDB database (PDB ID: 3HMU) and the sulfate ions in active sites were replaced with PMP to construct the holoenzyme. A galactomannan oligosaccharide with an 11-mannose backbone and one galactose side chain on the fifth mannose unit connected via α-(1→6)-linkage was used in the docking simulation