Fig. 3
Kinetic parameters of wild-type and mutant TLL determined using a series of pNPP concentrations (10 mM to 100 mM) at 10 mM intervals as a substrate. a Catalytic efficiency (kcat/Km), and b Km values. The error bars in graphs represent standard deviations (n = 3). The symbols indicate significant differences between mutants and wild-type: ns (no significant difference), p > 0.05; *, p < 0.05; **, p < 0.01