Fig. 5

Comparison of enzymatic properties between wt–TLL and positive mutants (M11 and M21). a Optimum temperature for wt–TLL and the mutants M11 and M21. b Optimal pH for wt–TLL and the mutants M11 and M21. c The methanol tolerance of wt–TLL and the mutants M11 and M21 determined by analyzing the residual activity of enzymes preincubated in methanol at various concentrations (10% to 90%) for 1 h at their respective optimum temperatures. The error bars in graphs represent standard deviations (n = 3). The symbols indicate significant differences between mutants and wild-type: ns (no significant difference), p > 0.05; *, p < 0.05; **, p < 0.01