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Fig. 6 | Biotechnology for Biofuels and Bioproducts

Fig. 6

From: In silico design of multipoint mutants for enhanced performance of Thermomyces lanuginosus lipase for efficient biodiesel production

Fig. 6

Comparison of the FAME yield between wt–TLL and positive mutants (M11 and M21). a Transesterification of soybean oil at 45, 50, and 55 °C after 48 h. b Transesterification of soybean oil at 50 °C for different times (12, 24, and 48 h). The error bars in graphs represent standard deviations (n = 3). The symbols indicate significant differences between mutants and wild-type: ns (no significant difference), p > 0.05; *, p < 0.05; **, p < 0.01

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