Fig. 8

Structural analysis of the interaction between the main contents of soybean oil (palmitic, oleic and linoleic acids) and the mutant amino-acid residues in the binding site of wt–TLL, the mutant M11, and the mutant M21. a wt–TLL, pNPP; b wt–TLL, palmitic acid; c wt–TLL, oleic acid; d wt–TLL, linoleic acid; e M11, pNPP; f M11, palmitic acid; g M11, oleic acid; h M11, linoleic acid; i M21, pNPP; j M21, palmitic acid; k M21, oleic acid; l M21, linoleic acid. The catalytic S146 are colored in green and shown as sticks. The target residues for mutagenesis are colored in yellow and shown as sticks. The substrates are colored in cyan and shown as ball and sticks. The blue dashed line indicates the hydrophilic attack distance. The magenta dashed line indicates the interaction