Table 2 Overview of previous studies on use of lipases in biodiesel production
Organism | Expression system | Lipase | Biodiesel feedstock | Yield (%) | Ratio (oil:methanol) | Temperature (°C) | Time (h) | References |
|---|---|---|---|---|---|---|---|---|
Thermomyces lanuginosus | Pichia pastoris KM71H | G91C | Cornus wilsoniana oil | 91.00 | 1:5 | 43 | 60 | [30] |
Thermomyces lanuginosus | Escherichia coli | S105C/D27R | Waste grease | 90.00 | 1:3 | 30 | 24 | [29] |
Rhizomucor miehei | Pichia pastoris GS115 | N314 | Waste soybean oil | 70.50 | 1:6 | 35 | 24 | [55] |
Rhizomucor miehei | Pichia pastoris GS115 | N267 | Waste soybean oil | 81.70 | 1:6 | 35 | 24 | [15] |
Rhizomucor miehei | Pichia pastoris GS115 | N218 | Colza oil | 90.46 | 1:6 | 35 | 24 | [56] |
Geobacillus stearothermophilus | Escherichia coli BL21 | A187F/L360F | Waste chicken oil | 88.00 | 1:5 | 45 | 24 | [16] |
Geobacillus stearothermophilus | Escherichia coli BL21 | H86Y/A269T/R374W | Waste chicken oil | 64.00 | 1:4.5 | 45 | 24 | [57] |
Yarrowia lipolytica | Escherichia coli BL21 | YLIP9L1Bp3 | Palm oil | ~ 40.00 | 1:5 | 50 | 24 | [58] |
Yarrowia lipolytica | Yarrowia lipolytica | lipase 2 | Soybean oil | 91.80 | 1:4 | 40 | 24 | [59] |
Thermomyces lanuginosus | Pichia pastoris GS115 | M21 | Soybean oil | 98.56 (93.37) | 1:4 | 50 | 48 (24) | This study |