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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Identification of environment-insensitive genes for oil content by combination of transcriptome and genome-wide association analysis in rapeseed

Fig. 2

Summary of DEGs between the extremely HOC and LOC inbred lines. Number of stable up-regulated (a) and down-regulated (b) DEGs in CS, HZ and KM. c The heatmap of 39 selected stable DEGs from the comparison between HOC and LOC inbred lines in three different environments. Three biological replicates were used to calculate expression values with three technical replicates, and normalized by log10(mean expression values). d Transcriptome sequencing verified by qRT-PCR. Normalized expression (2−∆∆Ct) in HOC inbred lines was divided by normalized expression in LOC inbred lines and log2 transformed. The correlation coefficient (R2) was calculated for comparison. The orange, green and turquoise lines represent the CS, HZ and KM environment fitted curves, respectively. p values show the significance of pairwise comparisons

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