Table 1 List of primers used for construction of suicide plasmids for knockout of the genes coding for PQQ-mDHs
Primer name | Template | Target locus | Primer sequence (5'—3') | Reference |
|---|---|---|---|---|
CmR_FW | KTK_137 | Chloramphenicol resistance cassette | ttgatcgggcacgtaTGATCGGCACGTAAGAGGTTCCAAC | This work |
CmR_RV | KTK_137 | Chloramphenicol resistance cassette | accaataaaaaacgcAGCGGAAAAGGACAAAAGTCA | This work |
pUC_FW | KTK_354 | Plasmid backbone (pUC19-based) | catgtgagcaaaaggccagcaa | This work |
pUC_RV | KTK_354 | plasmid backbone (pUC19-based) | tatggtgcactctcagtacaatctgct | This work |
KS003-UF | KS001 gDNA | CFR77_05025 | tgagagtgcaccataATGAATAGCCTCATACGCTCG | This work |
KS003-UR | KS001 gDNA | CFR77_05025 | tacgtgcccgatcaaTGTCCAGGCCACCTTCAG | This work |
KS003-DF | KS001 gDNA | CFR77_05025 | gcgttttttattggtGCAATCTCGGCATGTTCGAA | This work |
KS003-DR | KS001 gDNA | CFR77_05025 | ccttttgctcacatgTCAGTTCCCGTCAGGCAGG | This work |
KS002-UF | KS001 gDNA | CFR77_01730 | tgagagtgcaccataATGCGAGAAACCACCAAGAGG | This work |
KS002-UR | KS001 gDNA | CFR77_01730 | tacgtgcccgatcaaGACTTCAAGGTTGAACTCATGACCC | This work |
KS002-DF | KS001 gDNA | CFR77_01730 | gcgttttttattggtTCATGCTGGCCAATGCGAG | This work |
KS002-DR | KS001 gDNA | CFR77_01730 | ccttttgctcacatgTTATTGGGCTGCATTGCCTGCC | This work |
KS004-UF | KS001 gDNA | CFR77_00430 | tgagagtgcaccataATGATTTCTGCCGTTTTCGGAAAAAGACG | This work |
KS004-UR | KS001 gDNA | CFR77_00430 | tacgtgcccgatcaaGCCATCAACCGTGTAGGAACGC | This work |
KS004-DF | KS001 gDNA | CFR77_00430 | gcgttttttattggtGTCTGGCCAACGGCGAAT | This work |
KS004-DR | KS001 gDNA | CFR77_00430 | ccttttgctcacatgTTATGGCTGCTGCTCGGGAATACCG | This work |
KS005-UF | KS001 gDNA | CFR77_09325 | tgagagtgcaccataATGCTGCGCACTCTGCTG | This work |
KS005-UR | KS001 gDNA | CFR77_09325 | tacgtgcccgatcaaGGAAGTGTAATACACCACCGCCTTACAC | This work |
KS005-DF | KS001 gDNA | CFR77_09325 | gcgttttttattggtCGTATGACGAAAAGACCGGTATCCTGA | This work |
KS005-DR | KS001 gDNA | CFR77_09325 | ccttttgctcacatgTCAGTTCACGTCGTCCAGCGC | This work |