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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity

Fig. 2

Transformation efficiency of strain NIES-2152. a Transformation efficiencies at different electric-field strengths with various pulse durations of Pp. The electric-field strength, number of pulses, and pulse duration of Tp were fixed at 100 V cm−1, 5 pulses, and 50 ms, respectively. b Effect of electric-field strength of Tp on transformation efficiency. The electric-field strength and pulse duration of Pp were fixed at 2000 V cm−1 and 9 ms, respectively. The transformation efficiency was calculated by dividing the number of ZeocinⓇ-resistant colonies by the number of input cells (3.0 × 107)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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