Fig. 3
Construction and characterization of CDMT1-knockout strains. a PCR detection of a bleHH insertion into a crRNA target site. M: molecular size markers (λ-EcoT14 I digest), PCR product amplified with DNA from W: strain NIES-2152, CR24: strain CR24, CR26: strain CR26, and N: no DNA. b The target sequence of CDMT1_3 crRNA and the corresponding sequences in the knockout strains, CR24 and CR26. Each broad orange arrow represents one unit length of bleHH. A Δ symbol followed by a number indicates a deletion of bases with this number at one end of bleHH. Inserted nucleotide(s) are shown in red. c, d Cells of the wild-type strain, strain PK4, and CDMT1-knockout strains were grown in 1/5 UP medium under continuous light for 4 to 14 days. c Volumetric biomass yield [cell mass dry weight per liter of culture (g l−1)]. d Lipid content in percentage of dry weight biomass (w/w). Bars represent standard deviations of data from three independent samples. Statistical significance of differences between the values of strain PK4 and those of other strains were tested by Student’s t test (two-tailed), and the results are shown as asterisks. Single asterisks indicate P values between 0.01 and 0.05, and double asterisks indicate P < 0.01