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Table 1 Sequence analysis of EcTDH, EhNOX, EcSstT, and ScAAO

From: Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration

Enzyme

Theoretical pI

Theoretical molecular weight (Da)

Instability index II

Half-lives in E. coli (h)

Half-lives in yeast (h)

EcTDH

5.81

37173.94

24.46

 > 10

 > 20

EhNOX

4.88

49314.96

26.96

 > 10

 > 20

EcSstT

8.81

43507.72

35.19

 > 10

 > 20

ScAAO

6.93

42678.90

31.52

 > 10

 > 20