Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration

Table 2 Enzyme activity of TDH and NOX from different engineered E. coli

Strains	Enzyme activity(IU/mL)
Strains	TDH	NOX
E. coli BL21/pACYCDuet-1	0.31	0.26
E. coli BL21/pACYCDuet-1-Ectdh	25.32	0.32
E. coli BL21/pACYCDuet-1-Ectdh-Ehnox	24.82	2.83
E. coli BL21/pACYCDuet-1-Ectdh-Ehnox: pETDuet-1-Scaao	23.20	2.49
E. coli BL21/pACYCDuet-1-Ectdh-Ehnox: pETDuet-1-Scaao-Ecsstt	18.55	2.38
E. coli BL21/pETDuet-1	0.27	0.35

ISSN: 2731-3654