Table 1 Kinetic parameters of PobA mutants in different studies
From: Exploring the role of flavin-dependent monooxygenases in the biosynthesis of aromatic compounds
Variants | 4-HBA | 3,4-DHBA | References | ||||
|---|---|---|---|---|---|---|---|
KM | kcat | kcat/KM | KM | kcat | kcat/KM | ||
(μM) | (s-1) | (μM−1 s-1) | (μM) | (s-1) | (μM−1 s-1) | ||
Wide type | 34.67 ± 9.51 | 14.12 ± 1.49 | 0.41 | – | – | – | [47] |
Y385F | 19.55 ± 0.32 | 0.20 ± 0.001 | 0.01 | 228.02 ± 53.57 | 0.39 ± 90.06 | 0.0002 | |
Y385F/T294A | 48.38 ± 3.00 | 0.90 ± 0.03 | 0.02 | 157.02 ± 31.41 | 1.69 ± 0.18 | 0.012 | |
Y385F/L199V | 22 ± 5 | 0.45 ± 0.02 | 0.021 | 52 ± 16 | 4.4 ± 0.4 | 0.085 | [49] |
L199R/T294C/Y385M | 24 ± 10 | 1.9 ± 0.2 | 0.079 | 48 ± 10 | 3.0 ± 0.2 | 0.062 | [56] |
V47I/L199N/T294A/Y385I | 120 ± 10 | 4.0 ± 0.1 | 0.034 | 120 ± 10 | 4.1 ± 0.2 | 0.033 | |
Y385F/T294A/V349A | 14.5 ± 2.5 | 1.36 ± 0.04 | 0.094 | 30.3 ± 10.4 | 1.78 ± 0.16 | 0.059 | [57] |