Table 2 Kinetic parameters of PsAaoA on aryl alcohols and substrate conversion after 8 h incubation
From: Substrate specificity mapping of fungal CAZy AA3_2 oxidoreductases
Substrate | KM (mM) | kcat (s−1) | kcat/KM (M−1.s−1) | Degree of oxidation (%) |
|---|---|---|---|---|
benzyl alcohol | 1.6 ± 0.7 | 12.4 ± 1.5 | (7.8 ± 3.7) × 103 | 29 |
4-hydroxybenzyl alcohol | 9.9 ± 2.0 | 2.1 ± 0.2 | 209 ± 47 | 29 |
p-anisyl alcohol | 0.47 ± 0.06 | 4.7 ± 0.1 | (1.0 ± 0.1) × 104 | 64 |
4-hydroxy-3-methoxybenzyl alcohol | 8.7 ± 1.6 | 1.7 ± 0.1 | 200 ± 40 | 47 |
3,4-dimethoxybenzyl alcohol | 4.0 ± 1.3 | 1.1 ± 0.1 | 269 ± 93 | 56 |
3,5-dimethoxy-4-hydroxybenzyl alcohol (syringyl alcohol) | n.m. | n.m. | n.m. | 3 |
cinnamyl alcohol | 0.05 ± 0.01 | 10.5 ± 0.4 | (2.2 ± 0.4) × 105 | 89 |
p-coumaryl alcohol | 0.15 ± 0.02 | 3.3 ± 0.02 | (2.1 ± 0.3) × 104 | 74 |
coniferyl alcohol | 0.14 ± 0.01 | 4.7 ± 0.1 | (3.2 ± 0.3) × 104 | 84 |
sinapyl alcohol | n.m. | n.m. | n.m. | 5 |
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