Table 3 Kinetic parameters of ApGoxA with carbohydrate substrates
From: Substrate specificity mapping of fungal CAZy AA3_2 oxidoreductases
Substrate | ApGoxA-Oxygen | ApGoxA-BQ | ||||
|---|---|---|---|---|---|---|
KM (mM) | kcat (s−1) | kcat/KM (M−1.s−1) | KM (mM) | kcat (s−1) | kcat/KM (M−1.s−1) | |
Glucose | 7.3 ± 0.5 | 15.5 ± 0.5 | (2.1 ± 0.2) × 103 | 2.9 ± 0.1 | 41.4 ± 0.8 | (1.5 ± 0.6) × 104 |
Xylose | 81.3 ± 4.4 | 3.1 ± 0.1 | 38 ± 2 | 105.5 ± 14.9 | 3.1 ± 0.2 | 29 ± 10 |
Mannose | 435.6 ± 23.4 | 9.2 ± 0.2 | 21 ± 2 | (1.4 ± 0.1) × 103 | 21.6 ± 0.8 | 15 ± 10 |
Gentiobiose | n.m. | n.m. | ||||