Table 4 Kinetic parameters of TaGdhA and KiOdhA with carbohydrate substrates
From: Substrate specificity mapping of fungal CAZy AA3_2 oxidoreductases
Substrate | TaGdhA | KiOdhA | ||||
|---|---|---|---|---|---|---|
KM (mM) | kcat (s−1) | kcat/KM (M−1.s−1) | KM (mM) | kcat (s−1) | kcat/KM (M−1.s−1) | |
Glucose | 3.1 ± 0.2 | 92.5 ± 1.9 | (3.0 ± 1.0) × 104 | 69.0 ± 11.3 | 9.0 ± 0.8 | 130 ± 68 |
Xylose | 1.2 ± 0.1 | 24.3 ± 0.2 | (2.0 ± 0.6) × 104 | n.m. | ||
Mannose | 524.8 ± 19.2 | 36.4 ± 0.5 | 69 ± 25 | n.m. | ||
Gentiobiose | 1.7 ± 0.8 | 1.4 ± 0.2 | 818 ± 290 | 3.1 ± 0.7 | 10.7 ± 1.2 | (3.5 ± 1.5) × 103 |