Structural insights into curdlan degradation via a glycoside hydrolase containing a disruptive carbohydrate-binding module

Table 1 Specific activities of CBM6E WT and rational design variants using curdlan high-set gel at 15 mg/mL concentration as substrate

^aOne unit of enzyme activity corresponds to 1 μmol of glucose released in 1 min. Measurements were conducted in triplicate for each enzyme at 35 °C, using a buffer solution consisting of 50 mM Na₂HPO₂–KH₂PO₄ at pH 6.0. The reported values represent the means accompanied by their respective standard deviations
^bThe specific activity of CBM6E WT is from the reference [19]

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