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Table 3 Quantitative binding assay to curdlan high-set gel of CBM6E-CBM6 WT and its variants targeting potential sugar binding sites

From: Structural insights into curdlan degradation via a glycoside hydrolase containing a disruptive carbohydrate-binding module

Protein name

Kd (mg/mL)

CBM6E-CBM6-WT

0.9 ± 0.2

CBM6E-CBM6-V435A

0.9 ± 0.2

CBM6E-CBM6-I382A

3.0 ± 1.6

CBM6E-CBM6-T376A

24.4 ± 1.8

CBM6E-CBM6-V388A

18.8 ± 1.5

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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