Fig. 3From: Insights into the action of phylogenetically diverse microbial expansins on the structure of cellulose microfibrilsImpact of microbial expansins on hydrolytic and oxidative enzymes used to treat hardwood kraft pulp. The reactions were performed at 40 °C, pH 5.5 up to 72 h. Soluble sugars released were measured in the presence of a Endoglucanase (EG) + microbial expansin (EXLX); b Xylanase (Xyl) + EXLX; c TrAA9A + EXLX. The first, most left column in each panel shows product release by a Endoglucanase (EG), b Xylanase, and c TrAA9A alone. Addition of BSA instead of expansin was used in each case as a reference. Total reducing sugars released in the hydrolysis of pulp by endoglucanase and xylanase after 5/6, 24, 48, and 72 h reaction time were measured using the PAHBAH assay (a and b). In the case of the oxidative TrAA9A enzyme (c), the soluble products were analyzed using UHPLC-IMS-MS at the 72 h time point. Error bars indicate standard deviations for triplicate samples. Asterisks (*) indicate statistically significant (p ≤ 0.05; two-tailed t-test) difference between EXLX and BSA-treated samplesBack to article page