Fig. 2

Subcellular localization of sfGFP-TrRab7 under cellulase induction condition. A Spherical structures (white triangle) observed in fluorescence microscopy analysis of sfGFP-TrRab7; B Distinct motile punctate structures (arrows) corresponding to pre-vacuolar compartments (PVCs) observed in fluorescence microscopy analysis of sfGFP-TrRab7. The strain was grown in MA medium with 1% (w/v) Avicel and mycelia were collected and stained with 10 μM FM4-64 for fluorescence microscopy analysis at 24 h. The fluorescence was examined with a Nikon Eclipse 80i fluorescence microscope. Scale bar: 10 µm. The representative images shown were taken from at least two independent experiments