Fig. 4

Trrab7 knock-down compromised the cellulase production of T. reesei. A Hydrolytic zone formation by the QM9414 and Ptcu-rab7^KD strains on MA agar plates covered with a 0.4% (w/v) ground Avicel layer. Extracellular pNPC (B), pNPG (C), CMCase (D), xylanase (E) activities, and protein concentration (F) of the culture supernatant of strains cultured using 1% (w/v) Avicel with or without copper for the indicated periods. G SDS–PAGE analysis of the culture supernatant of QM9414 and Ptcu-rab7^KD with 1% (w/v) Avicel. Culture supernatants (20 μL) were taken at indicated time points and loaded for analysis. M denotes standard molecular weight protein marker. Significant differences were determined by a two-tailed student's t test. *, P < 0.05; **P < 0.01; *** P < 0.001