Fig. 5

Trrab7 knock-down showed no large effect on the secretion of overexpressed EG1. A Extracellular CMCase activities of the culture supernatant from QM9414, QM9414 & Pcdna-eg1, and Ptcu-rab7^KD & Pcdna-eg1 strains cultured with 1% (w/v) glucose at indicated time points. Significant differences were determined by a two-tailed student's t test. *, P < 0.05; **P < 0.01; ***P < 0.001. B SDS–PAGE analysis of the culture supernatants of indicated strains cultured with 1% (w/v) glucose. M denotes standard molecular weight protein marker. Culture supernatants (20 μL) were taken at indicated time points and loaded for analysis. As the parental strains QM9414 and Ptcu-rab7^KD did not produce significant extracellular proteins with glucose as the sole carbon source, the observed protein band with the highest intensity on SDS–PAGE between 55 and 70 kD was EG1