Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus

Table 1 Effect of milling time on enzymatic digestibility of eucalyptus in ball-milling treatment

Milling time (minutes)	Yields of hydrolyzed sugars		Total yield (%)	CrI^a (%)
	Glucose (%)	Xylose (%)
0	-	-	-	59.7
10	16.0	20.5	16.9	25.9
20	46.8	34.3	44.2	7.6
40	70.4	49.2	66.0	3.0
120	89.7	72.5	86.2	6.4

The ball-milling (BM)-treated sample (1%) was hydrolyzed with an enzyme cocktail (40 FPU/g substrate) for 72 hours as described in materials and methods. BM treatment was performed using pulverisette 7. The hydrolysis yields of glucose and xylose are calculated based on the glucan and xylan contents, respectively, in the dry weight of untreated biomass. Total yield of hydrolyzed sugars is calculated from the combined contents of glucan and xylan in the dry weight of untreated biomass. ^aCrystallinity index of the BM-treated sample.

ISSN: 2731-3654