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Table 2 Langmuir and kinetic adsorption parameters of purified cellulases using α-cellulose at n = 1000 rpm

From: Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics

Cellulase Langmuir adsorption parameters* Kinetic adsorption parameters
  A max [μmol/g] K D [μmol/L] [-] A max [μmol/g] k ad [s -1 ] [-]
CBH I 0.155 ± 0.003 0.433 ± 0.039 0.93 0.170 ± 0.003 0.0031 ± 0.0002 0.98
EG I 0.212 ± 0.010 2.146 ± 0.216 0.90 0.213 ± 0.007 0.0019 ± 0.0002 0.96
  1. * According to Eqn (1).
  2. According to Eqn (2).
  3. Differences of A max resulted because different charges of CBH I were used.
  4. Errors are given as standard deviations.
  5. A max , the maximum cellulase adsorption at equilibrium; K D , the dissociation constant; k ad , the pseudo-first-order adsorption rate constant; CBH, cellobiohydrolase; EG, endoglucanase.