Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics

Table 2 Langmuir and kinetic adsorption parameters of purified cellulases using α-cellulose at n = 1000 rpm

Cellulase	Langmuir adsorption parameters*			Kinetic adsorption parameters^†
	A _max [μmol/g]	K _D [μmol/L]	R² [-]	A _max [μmol/g]	k _ad [s ^-1 ]	R² [-]
CBH I	0.155^‡ ± 0.003	0.433 ± 0.039	0.93	0.170^‡ ± 0.003	0.0031 ± 0.0002	0.98
EG I	0.212 ± 0.010	2.146 ± 0.216	0.90	0.213 ± 0.007	0.0019 ± 0.0002	0.96

* According to Eqn (1).
^† According to Eqn (2).
^‡ Differences of A_max resulted because different charges of CBH I were used.
Errors are given as standard deviations.
A_max, the maximum cellulase adsorption at equilibrium; K_D, the dissociation constant; k_ad, the pseudo-first-order adsorption rate constant; CBH, cellobiohydrolase; EG, endoglucanase.

ISSN: 2731-3654