Figure 1
From: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity
HPAEC-PAD analysis of 1 g/L low xylan oligosaccharide and 1 g/L high xylan oligosaccharide. High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis of low xylan oligosaccharide (XOS, open columns) and high XOS (grey columns). The peak from xylobiose to xylopentose was identified by comparison with commercially available XOSs, and longer oligosaccharides were named accordingly. The peak areas of 10 μL of 5 g/L solutions are shown. The response factors for xylobiose to xylopentose were between 0.09 and 0.1 and were almost constant. The average degree of polymerisation (DP) (by peak area) of low XOS was 4.0, 80% between (xyl)2 and (xyl)6 and 90% between (xyl)2 and (xyl)7; the average DP of high XOS was 8.1, 80% between (xyl)5 and (xyl)13 and 90% between (xyl)3 and (xyl)13.