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Table 3 Para-nitrophenyl release of TrCel7A and TrCel7A core from pNP-lac as a single substrate at 50°C and 30°Ca

From: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity

Enzyme (temperature
substrate)
Activity in 1 hour Activity in 1 hour (1 g/L low XOS) Activity in 1 hour (1 g/L high XOS)
TrCel7A core, 50°C 127 ± 5 μM pNPb
436 ± 11 μM cbb
21% ± 4 pNP
61% ± 6 cb
10% ± 2 pNP
44% ± 2 cb
TrCel7A, 50°C 99 ± 3 μM pNP
695 ± 32 μM cb
15% ± 2 pNP
96% ± 4 cb
9% ± 1 pNP
81% ± 5 cb
TrCel7A core, 30°C 50 ± 2 μM pNP
177 ± 6 μM cb
21% ± 2 pNP
61% ± 7 cb
10% ± 4 pNP
55% ± 4 cb
TrCel7A, 30°C 38 ± 1 μM pNP
204 ± 10 μM cb
15% ± 3 pNP
96% ± 2 cb
9% ± 3 pNP
81% ± 2 cb
  1. cb: cellobiose-solubilised; pNP = para-nitrophenyl release; pNP-lac = para-nitrophenyl-β-D-lacoside; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide. aThe cellobiose release from phosphoric acid swollen cellulose (PASC) in the presence of pNP-lac by TrCel7A and TrCel7A core at 50°C and 30°C was measured in separate experiments. The deviations are the standard deviations of five independent experiments. bThe cellobiose release from PASC was independent of pNP-lac (Table 4).