Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity

Table 3 Para-nitrophenyl release of TrCel7A and TrCel7A core from pNP-lac as a single substrate at 50°C and 30°C^a

Enzyme (temperature substrate)	Activity in 1 hour	Activity in 1 hour (1 g/L low XOS)	Activity in 1 hour (1 g/L high XOS)
TrCel7A core, 50°C	127 ± 5 μM pNP^b 436 ± 11 μM cb^b	21% ± 4 pNP 61% ± 6 cb	10% ± 2 pNP 44% ± 2 cb
TrCel7A, 50°C	99 ± 3 μM pNP 695 ± 32 μM cb	15% ± 2 pNP 96% ± 4 cb	9% ± 1 pNP 81% ± 5 cb
TrCel7A core, 30°C	50 ± 2 μM pNP 177 ± 6 μM cb	21% ± 2 pNP 61% ± 7 cb	10% ± 4 pNP 55% ± 4 cb
TrCel7A, 30°C	38 ± 1 μM pNP 204 ± 10 μM cb	15% ± 3 pNP 96% ± 2 cb	9% ± 3 pNP 81% ± 2 cb

cb: cellobiose-solubilised; pNP = para-nitrophenyl release; pNP-lac = para-nitrophenyl-β-D-lacoside; TrCel7A = cellobiohydrolase I; XOS = xylan oligosaccharide. ^aThe cellobiose release from phosphoric acid swollen cellulose (PASC) in the presence of pNP-lac by TrCel7A and TrCel7A core at 50°C and 30°C was measured in separate experiments. The deviations are the standard deviations of five independent experiments. ^bThe cellobiose release from PASC was independent of pNP-lac (Table 4).

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