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Figure 4 | Biotechnology for Biofuels

Figure 4

From: Product inhibition of cellulases studied with 14C-labeled cellulose substrates

Figure 4

Analysis of the inhibition of GH7 CBHs by cellobiose. (A) Data for the hydrolysis of 14C-BC by the mixture of TrCel7A and TrCel5A at 35°C (Figure 3A) were rearranged in the coordinates (DCB/DCB=0) versus [cellobiose], where DCB and DCB=0 represent the degree of conversion of 14C-BC in the presence and absence of cellobiose, respectively. The ratio of (DCB/DCB=0) was found after different times of hydrolysis, which were 2 min (), 5 min (□), 10 min (Δ), 20 min (), and 30 min (×). (B) Data for the hydrolysis of 14C-BC by the mixture of CBH and TrCel5A at 35°C (Figure 3) in the coordinates (DCB/DCB=0) versus [cellobiose]. (DCB/DCB=0) values for all hydrolysis time points are shown. CBH was TrCel7A (□), TaCel7A, (), AtCel7A (Δ), and CtCel7A (×). Solid lines are from the non-linear regression according to Equation 5.

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