Figure 4

Analysis of the inhibition of GH7 CBHs by cellobiose. (A) Data for the hydrolysis of ¹⁴C-BC by the mixture of TrCel7A and TrCel5A at 35°C (Figure 3A) were rearranged in the coordinates (D_CB/D_CB=0) versus [cellobiose], where D_CB and D_CB=0 represent the degree of conversion of ¹⁴C-BC in the presence and absence of cellobiose, respectively. The ratio of (D_CB/D_CB=0) was found after different times of hydrolysis, which were 2 min (◊), 5 min (□), 10 min (Δ), 20 min (○), and 30 min (×). (B) Data for the hydrolysis of ¹⁴C-BC by the mixture of CBH and TrCel5A at 35°C (Figure 3) in the coordinates (D_CB/D_CB=0) versus [cellobiose]. (D_CB/D_CB=0) values for all hydrolysis time points are shown. CBH was TrCel7A (□), TaCel7A, (◊), AtCel7A (Δ), and CtCel7A (×). Solid lines are from the non-linear regression according to Equation 5.