Table 1 Kinetic parameters for cellobiose hydrolysis by β-glucosidases
From: Selecting β-glucosidases to support cellulases in cellulose saccharification
kcat(h)(s-1)a | KM(h) (mM)a | |||||
|---|---|---|---|---|---|---|
t (°C) | N188BG | TaBG3 | AtBG3 | N188BG | TaBG3 | AtBG3 |
25 | 121 ± 4 (70%) | 227 ± 10 (57%) | 105 ± 11 (95%) | 0.73 ± 0.04 | 0.42 ± 0.03 | 0.29 ± 0.07 |
35 | 271 ± 5 (72%) | 401 ± 19 (57%) | 180 ± 11 (92%) | 0.97 ± 0.04 | 0.51 ± 0.04 | 0.32 ± 0.04 |
45 | 493 ± 12 (70%) | 632 ± 20 (61%) | 326 ± 14 (81%) | 1.34 ± 0.06 | 0.60 ± 0.03 | 0.41 ± 0.04 |
55 | 691 ± 27 (76%) | 1058 ± 81 (60%) | 666 ± 23 (66%) | 1.36 ± 0.12 | 0.67 ± 0.10 | 0.87 ± 0.05 |
65 | 1497 ± 53 (67%) | 968 ± 31 (71%) | 0.82 ± 0.06 | 0.93 ± 0.06 | ||