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Table 1 Kinetic parameters for cellobiose hydrolysis by β-glucosidases

From: Selecting β-glucosidases to support cellulases in cellulose saccharification

  kcat(h)(s-1)a KM(h) (mM)a
t (°C) N188BG TaBG3 AtBG3 N188BG TaBG3 AtBG3
25 121 ± 4 (70%) 227 ± 10 (57%) 105 ± 11 (95%) 0.73 ± 0.04 0.42 ± 0.03 0.29 ± 0.07
35 271 ± 5 (72%) 401 ± 19 (57%) 180 ± 11 (92%) 0.97 ± 0.04 0.51 ± 0.04 0.32 ± 0.04
45 493 ± 12 (70%) 632 ± 20 (61%) 326 ± 14 (81%) 1.34 ± 0.06 0.60 ± 0.03 0.41 ± 0.04
55 691 ± 27 (76%) 1058 ± 81 (60%) 666 ± 23 (66%) 1.36 ± 0.12 0.67 ± 0.10 0.87 ± 0.05
65   1497 ± 53 (67%) 968 ± 31 (71%)   0.82 ± 0.06 0.93 ± 0.06
  1. The values in parentheses show the highest measured value of the rate constant for cellobiose hydrolysis as a percentage of kcat(h).
  2. aThe values of kcat(h) and KM(h) were determined by a non-linear regression analysis of the data of cellobiose turnover, according to Equation 2.