Figure 11
![Figure 11](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1754-6834-6-120/MediaObjects/13068_2013_Article_344_Fig11_HTML.jpg)
Inhibitor tolerance assay in acid-pretreated spruce hydrolysate with the three new hybrid strains in a small-scale fermentation experiment. The fermentation was performed using the whole slurry of spruce hydrolysate (60%) supplemented with 40 g/L glucose with continuous stirring at 200 rpm. Cells were inoculated at an initial cell density of 1.3 g DW/L from a stationary phase preculture in YPD medium. The CO2 production was estimated from the weight loss during fermentation.