Figure 6

Pyruvate decarboxylase activity and α-ketoacids bioconversion into butanol and isobutanol. (A) α-ketovalerate and α-isoketovalerate conversion reaction performed by pyruvate decarboxylase enzyme. The carbons of the molecules were numbered. (B) Pyruvate decarboxylase deletion effect on butanol and isobutanol accumulation using α-ketovalerate as substrate. Wild type (empty column) and PCD1, 5, 6 deleted strains (full column). The data presented here are representative of three independent experiments. p ≤ 0,05 = *; p ≤ 0,01 = **; p ≤ 0,001 = ***; p > 0,05 = n.s. (C) α-Ketovalerate (left panel) and α-isoketovalerate (right panel) bioconversion. Butanol (dark grey), isobutanol (white) and α-ketovalerate consumption (triangle) were reported. For α-isoketovalerate (triangle), the sole estimated initial concentration is given. The data presented here are representative of three independent experiments.