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Figure 3 | Biotechnology for Biofuels

Figure 3

From: Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution

Figure 3

Schematic of the screening protocol for improved methanol tolerance by directed evolution of PML. (A) Steps for colony lift assay and staining protocol. (B) Hydrolysis of 1-napthyl palmitate and detection of 1-naphthol by fast blue to assay colonies for residual activity after exposure to methanol and heat. (C) A representative filter following step 9 in (A). Black arrows point to potential positives identified on the filter.

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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