Cellulase activity mapping of Trichoderma reesei cultivated in sugar mixtures under fed-batch conditions

Table 1 Reproducibility of the experimental design

Measurement	Unit	Mean value	Std. dev.	(RSD)
Specific protein production rate	mg_P g_X^-1 h^-1	11.9	± 1.0	(± 9%)
Specific cellulase activity	IU mg_P^-1	0.47	± 0.06	(± 10%)
Specific xylanase activity	IU mg_P^-1	130	± 20	(± 16%)
Specific endoglucanase activity	IU mg_P^-1	11.2	± 0.5	(± 4%)
Specific Cel7A (CBH I) activity	IU mg_P^-1	0.23	± 0.02	(± 8%)
Specific β-glucosidase activity	IU mg_P^-1	0.52	± 0.06	(± 10%)

The reproducibility of the whole experimental protocol (cell growth + protein production in fed-flask protocol + analyses) was assessed on the central point of the mixture design with lactose inducer (black circle Figure 1), by 10 repeats in 3 different series.

ISSN: 2731-3654