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Table 5 Laboratory evolution of xylose-fermenting strains of S. cerevisiae as tool for process intensification: comparison of key process parameters reported for the progenitor strain and the evolved strain, respectively

From: Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae

Progenitor strain - evolved strain

Fermentation conditionb

cSugar(g/L)c

qXylose(g/gCDW/h)

cEthanol(g/L)d

YEthanol(g/gtotal sugars)

μmax(h-1)e

Source

Genetic background a

TMB3001 -

MM

Glc: 50

0.08*

22*

0.38*

0.44Glc

[26]

TMB3001C1

AN

Xyl: 50

0.31*

28*

0.40*

0.44Glc

XR/XDH/XK

LCD

 

(3.9–fold)

(1.3–fold)

(1.1–fold)

(–)

H131-A3SB-2 -

YE

Xyl: 40

0.26

4*

0.42

0.06Xyl

[39]

H131-A3CS

AN

 

0.94

15*

0.43

0.12Xyl

XI/ PPP/ T

LCD

 

(3.6–fold)

(3.8–fold)

(–)

(2–fold)

HDY.GUF5 -

YE, Pep

Glc: 36

0.13

18*

0.23

n.a.

[23]

GS1.11-26

Semi-AN

Xyl: 37

1.10

34*

0.46

n.a.

XI/PPP

HCD

 

(8.5–fold)

(1.9–fold)

(1.8–fold)

(–)

BP10001 -

YE

Xyl: 50

0.37

0.9

0.30

-

This study

IBB10B05

AN

 

1.04

2.8

0.31

0.02Xyl

XR/XDH/XK

LCD

 

(2.8–fold)

(3.1–fold)

(–)

(–)

TMB3400 -

Wheat straw hydrolyzate, YE, salts, pH 5

Glc: 7.6

0.20*

5.5

0.20

-

[34]

KE6-13i

AN

Xyl: 38

0.04*

6

0.27

-

XR/XDH/XK

HCD

 

(-)

(1.1–fold)

(1.4–fold)

(–)

TMB3400 -

Spruce hydrolyzate, MM, pH 5

 

n.a.

7.8*

0.40

0.07Glc

[24]

KE1-17

AN

Glc: 18

n.a.

7.9*

0.43

0.08Glc

XR/XDH/XK

LCD

Xyl: 9

(–)

(–)

(1.1–fold)

(1.14–fold)

BP10001 -

Wheat straw hydrolyzate, YE, pH 6.5

 

0.02

4

0.40

0.10Glc

This study

IBB10B05

AN

Glc: 32

0.35

21

0.39

0.19Glc

XR/XDH/XK

HCD

Xyl: 16

(17.5–fold)

(5.3–fold)

(–)

(1.9–fold)

  1. aStrain background: PPP, overexpression of genes from the pentose phosphate pathway; T, overexpression of the HXT7 transporter; XDH, xylitol dehydrogenase; XI, xylose isomerase; XK, xylulose kinase; XR, xylose reductase. bAN, anaerobic; HCD, high cell density (start OD600 of fermentation ≥1); LCD, low cell density (start OD600 of fermentation ≤0.5); MM, mineral medium; Pep, peptone; YE, yeast extract. cInitial sugar concentration of the substrate. dEthanol produced within the first 50 h of fermentation or earlier, when sugars were depleted before. eMaximal growth rate on glucose (Glc) or xylose (Xyl). n.a., not analyzed; in parenthesis, improvement calculated from the evolved strain as compared to the progenitor strain; *Data are derived from the time courses given in the respective publications.