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Table 5 Laboratory evolution of xylose-fermenting strains of S. cerevisiae as tool for process intensification: comparison of key process parameters reported for the progenitor strain and the evolved strain, respectively

From: Process intensification through microbial strain evolution: mixed glucose-xylose fermentation in wheat straw hydrolyzates by three generations of recombinant Saccharomyces cerevisiae

Progenitor strain - evolved strain Fermentation conditionb cSugar(g/L)c qXylose(g/gCDW/h) cEthanol(g/L)d YEthanol(g/gtotal sugars) μmax(h-1)e Source
Genetic background a
TMB3001 - MM Glc: 50 0.08* 22* 0.38* 0.44Glc [26]
TMB3001C1 AN Xyl: 50 0.31* 28* 0.40* 0.44Glc
XR/XDH/XK LCD   (3.9–fold) (1.3–fold) (1.1–fold) (–)
H131-A3SB-2 - YE Xyl: 40 0.26 4* 0.42 0.06Xyl [39]
H131-A3CS AN   0.94 15* 0.43 0.12Xyl
XI/ PPP/ T LCD   (3.6–fold) (3.8–fold) (–) (2–fold)
HDY.GUF5 - YE, Pep Glc: 36 0.13 18* 0.23 n.a. [23]
GS1.11-26 Semi-AN Xyl: 37 1.10 34* 0.46 n.a.
XI/PPP HCD   (8.5–fold) (1.9–fold) (1.8–fold) (–)
BP10001 - YE Xyl: 50 0.37 0.9 0.30 - This study
IBB10B05 AN   1.04 2.8 0.31 0.02Xyl
XR/XDH/XK LCD   (2.8–fold) (3.1–fold) (–) (–)
TMB3400 - Wheat straw hydrolyzate, YE, salts, pH 5 Glc: 7.6 0.20* 5.5 0.20 - [34]
KE6-13i AN Xyl: 38 0.04* 6 0.27 -
XR/XDH/XK HCD   (-) (1.1–fold) (1.4–fold) (–)
TMB3400 - Spruce hydrolyzate, MM, pH 5   n.a. 7.8* 0.40 0.07Glc [24]
KE1-17 AN Glc: 18 n.a. 7.9* 0.43 0.08Glc
XR/XDH/XK LCD Xyl: 9 (–) (–) (1.1–fold) (1.14–fold)
BP10001 - Wheat straw hydrolyzate, YE, pH 6.5   0.02 4 0.40 0.10Glc This study
IBB10B05 AN Glc: 32 0.35 21 0.39 0.19Glc
XR/XDH/XK HCD Xyl: 16 (17.5–fold) (5.3–fold) (–) (1.9–fold)
  1. aStrain background: PPP, overexpression of genes from the pentose phosphate pathway; T, overexpression of the HXT7 transporter; XDH, xylitol dehydrogenase; XI, xylose isomerase; XK, xylulose kinase; XR, xylose reductase. bAN, anaerobic; HCD, high cell density (start OD600 of fermentation ≥1); LCD, low cell density (start OD600 of fermentation ≤0.5); MM, mineral medium; Pep, peptone; YE, yeast extract. cInitial sugar concentration of the substrate. dEthanol produced within the first 50 h of fermentation or earlier, when sugars were depleted before. eMaximal growth rate on glucose (Glc) or xylose (Xyl). n.a., not analyzed; in parenthesis, improvement calculated from the evolved strain as compared to the progenitor strain; *Data are derived from the time courses given in the respective publications.