Figure 5

Agronomic traits of early flowering Jatropha by JcFT over expression. (A) Flowering time in T0 Jatropha plant overexpressing JcFT. Flowering time was scored by the number of days from transplantation to soil to the day of first inflorescence emergence. Values are mean ± SD (n = 10). **indicate P < 0.01. (B) Correlation of early flowering (left panel) with JcFT expression level (right panel) in T1 Jatropha plants (#33 and #43).Values are mean ± SD (n = 3).**indicate P < 0.01. (C) JcFT overexpression reduces the time for flowering dormancy. The dormancy time is indicated by the numbers of leaves formed between the first and the second inflorescence. Values are mean ± SD (n = 3). **indicate P < 0.01. (D) Comparison of branch number of WT plant and two T1 JcFT overexpression lines (#33 and #43). Values are mean ± SE (n = 5). (E) Comparison of dry seed weight of WT and two T1 JcFT overexpression lines (#33 and #43). Values are mean ± SE (n = 10). (F) Comparison of seed number of WT plant and T0 JcFT overexpression lines (n = 4). (G) Comparison of seed number of WT plant and T1 JcFT overexpression line #43. * and ** indicate P < 0.05 and P < 0.01, respectively. Values are mean ± SE (n = 5). (H) Early flowering trait induced by JcFT overexpression was insensitive to low temperature. Flowering time was scored by the number of days from seed germination to the day of first inflorescence emergence. T1 transgenic plants of line #33 were used. Values are mean ± SD (n = 3). Null segregant derived from #33 (Null #33) was used as a control.