Figure 4

Cellulolytic activity (reducing sugars released by in vitro extracellular protein) of the extracellular fraction of Caldicellulosiruptor bescii grown on cellobiose, on carboxymethylcellulose (CMC) (gray bars) after 1 hour and Avicel (white bars) after 24 hours incubation with the extracellular fraction of wild-type, JWCB018 ( ΔpyrFA ΔcbeI ), and JWCB029 ( ΔpyrFA ΔcbeI ΔcelA ) strains.