Table 3 Primers used for PCR amplification of genes
Primer name | Primer sequence (5′-3′) |
|---|---|
ilvC-XbaI | GTATCTAGAAAGAGGAGATATAATGGCTAACTACTTCAATACACTGAATCTG |
ilvC-SpeI-BamHI | ACTGGATCCACTAGTTTAACCCGCAACAGCAATACGTT |
ilvD-XbaI | GTATCTAGAAAGAGGAGATATAATGCCTAAGTACCGTTCCGCCA |
ilvD-SpeI-BamHI | ACTGGATCCACTAGTTTAACCCCCCAGTTTCGATTTATCG |
BCKD-SacI | AGATGAGCTCATGGCAACTGAGTATGACGTAGTCATTC |
BCKD-SpeI | AGTACTAGTTTAGTAAACAGATGTCTTCTCGTCAATCG |
fabHB-BamHI | ACTGGATCCAAGAAGGAGATATAATGTCAAAAGCAAAAATTACAGCTATCGG |
fabHB-XhoI | TGTCTCGAGTTACATCCCCCATTTAATAAGCAATCCTG |
WS/DGAT-SfuI | AGGTTTCGAAATGAGACCATTACACCCTATTGATTTC |
WS/DGAT-EcoRI | GACGAATTCTTAGTTGGCAGTCTTAATGTCTTCTTG |
HIS4-BamHI | AATGGATCCCTCGCAGCTGATGAATATCTTGTG |
HIS4-BglII | CAAGGATCCACCGATACCAGGATCTTGCCAT |
ARO10-SfuI | GCGTTCGAAATGGCACCTGTTACAATTGAAAAGTTC |
ARO10-EcoRI | CCGTCTAGACTATTTTTTATTTCTTTTAAGTGCCGCTG |
ADH2-SfuI | CCGTTCGAAATGTCTATTCCAGAAACTCAAAAAGCC |
ADH2-EcoRI | GCGGAATTCTTATTTAGAAGTGTCAACAACGTATCTACCAG |