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Table 3 Kinetic parameters of chimeric enzymes compared with parental enzymes

From: Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

  Parental XynA 2091A 2621B
−Xylose +Xylose −Xylose +Xylose −Xylose +Xylose
K aM 1.5 ± 0.1 1.6 ± 0.1 1.7 ± 0.2 1.8 ± 0.1 2.1 ± 0.1 2.4 ± 0.2
k bcat 78 ± 2 70 ± 3 150 ± 8 200 ± 7 140 ± 5 240 ± 8
k cat/K M 52 ± 3 44 ± 1 89 ± 8 111 ± 3 67 ± 1 100 ± 7
  1. Data represent the mean ± SD of two independent preparations.
  2. amg mL−1.
  3. bs−1.