Table 3 Kinetic parameters of chimeric enzymes compared with parental enzymes
From: Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase
| Â | Parental XynA | 2091A | 2621B | |||
|---|---|---|---|---|---|---|
−Xylose | +Xylose | −Xylose | +Xylose | −Xylose | +Xylose | |
K aM | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.2 | 1.8 ± 0.1 | 2.1 ± 0.1 | 2.4 ± 0.2 |
k bcat | 78 ± 2 | 70 ± 3 | 150 ± 8 | 200 ± 7 | 140 ± 5 | 240 ± 8 |
k cat/K M | 52 ± 3 | 44 ± 1 | 89 ± 8 | 111 ± 3 | 67 ± 1 | 100 ± 7 |