Table 1 Strains used in this work
From: Engineering Yarrowia lipolytica to produce biodiesel from raw starch
Strains | Genotype or other relevant characteristics | Source or reference |
|---|---|---|
E. coli | ||
 DH5α | Φ80dlacZΔm15, recA1, endA1, gyrA96, thi-1, hsdR17 (r k −, m k +), supE44, relA1, deoR, Δ(lacZYA-argF)U169 | Promega |
Y. lipolytica | ||
 W29 | MATA, wild type | Barth and Gaillardin [48] |
 Po1d | MATA ura3-302 leu2-270 xpr2-322 | Barth and Gaillardin [48] |
 JMY2900 (WT) | Po1d Ura + Leu+ | Dulermo et al. [47] |
 JMY4926 | Po1d (Ura- Leu-) + pTEF-riceAlphaAmylase-URA3 | This work |
 JMY5077 (α) | JMY4926 + LEUex | This work |
 JMY4968 | Po1d + pTEF-Glucoamylase-URA3 | This work |
 JMY5083 (GA) | JMY4968 + LEUex | This work |
 JMY5017 (α + GA) | JMY4926 + pTEF-Glucoamylase-LEU2 | This work |
 JMY3501 | MATa ura3-302 leu2-270 xpr2-322 Δpox1-6 Δtgl4 + pTEF-DGA2-LEUex + pTEF-GPD1-URAex | Lazar et al. [41] |
 JMY3820 | MATa ura3-302 leu2-270 xpr2-322 Δpox1-6 Δtgl4 + pTEF-DGA2 + pTEF-GPD1 | Lazar et al. [41] |
 JMY4930 | JMY3820 + pTEF-riceAlphaAmylase-URA3 | This work |
 JMY5035 [JMY3820 (α + GA)] | JMY4930 + pTEF-Glucoamylase-LEU2 | This work |
 JMY5117 | JMY5035 (Ura- Leu-) | This work |
 JMY5196 [JMY3820 (α + GA) × 2] | JMY5117 pTEF-riceAlphaAmylase-URA3 + pTEF-Glucoamylase-LEU2 | This work |