Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica

Table 2 Biomass, citric acid, and lipid production

Parameter		X (g/L)	Y _X/S (g/g)	CA (g/L)	Y _CA/S (g/g)	Y _CA/X (g/g)	FA (g/L)	Y _FA/S (g/g)	Y _FA/X (g/g)
Flasks
Glu 6 % (C/N 60)	W29	11.15	0.24	4.18	0.09	0.37	1.92	0.041	0.172
Glu 6 % (C/N 60)	Y4588	10.90	0.21	18.17	0.35	1.67	1.87	0.036	0.172
Gal 6 % (C/N 60)		11.27	0.19	12.58	0.22	1.12	1.98	0.034	0.176
Gal 6 % (C/N 100)		8.45	0.17	16.96	0.35	2.01	1.79	0.037	0.212
Bioreactors
Gal 6 % (C/N 100)	Y4588	19.4	0.34	29.2	0.51	1.51	3.22	0.056	0.166

Y. lipolytica W29 and Y4588 strains were grown for 96 h in YNB medium containing 6 % glucose or 6 % galactose in flasks and bioreactors
In this analysis, SD did not exceed 7.5 %
X dry biomass, FA fatty acids, CA citric acid, Y _X/S yield of biomass from substrate consumed, Y _CA/S yield of citric acid from substrate consumed, Y _CA/X yield of citric acid from dry biomass, Y _FA/S yield of fatty acids from substrate consumed, Y _FA/X yield of fatty acids from dry biomass

ISSN: 2731-3654