Fig. 4
Figure adapted from [32]
a Pulsed electric field effects on the Nicotiana Tabacum L. cv bright yellow-2 cells with cell wall stained with vital dye solution (Phenosafranine). The pulsed electric field protocol: E = 2. 5 kV/cm, n = 20, f (pulse frequency) = 2 Hz, exponential pulses with duration of 400 ms. b Pulsed electric fields effects on the extracellular matrix of potato. The pulsed electric field protocol: E = 5 kV/cm, n = 20, f (pulse frequency) = 2 Hz, exponential pulses with duration of 100 ms. Tissue staining was performed with ruthenium red 5 min after PEF treatment