Table 4 CoGH1A and some reported microbial β-glucosidases with relatively high activity
Resource | GH family | Substrate | K m (mM) | V max (μmol mg−1 min−1) | k cat (s−1) | k cat/K m (mM−1 s−1) | OT(°C) | OP | References |
|---|---|---|---|---|---|---|---|---|---|
C. owensensis | GH1 | pNPGlu | 1.52 | 4027 | 3750.6 | 2467.5 | 75–85 | 5.5 | This work |
Cellobiose | 15.65 | 2424 | 2149 | 137.3 | |||||
C. bescii | GH1 | pNPGlu | 3.71 | – | 311.6 | 84.0 | 85 | 6.8 | [9] |
Cellobiose | 90.8 | – | 757.9 | 8.35 | |||||
Neosartorya fischeri | GH1 | pNPGlu | 68 | 886 | 826.7 | 12.2 | 40 | 6 | [46] |
Pholiota adipose | GH3 | pNPGlu | 2.23 | 4390 | – | – | 65 | 5 | [28] |
Cellobiose | 5.60 | 3460 | – | – | |||||
Fomitopsis pinicola | GH3 | pNPGlu | 1.8 | 1710 | 2990 | 1700 | 50 | 4.5 | [47] |
Thermotoga petroph | GH1 | pNPGlu | 2.8 | 42,700 | 87,400 | 30,800 | 80–90 | 7–8 | [10] |
Stereum hirsutum | GH1 | pNPGlu | 2.5 | 3028 | – | – | 65 | 4.5 | [48] |
Cellobiose | 86 | 172 | – | – | |||||
Talaromyces thermophilus | – | pNPGlu | 0.25 | 228.7 | – | – | 65 | 5.0 | [49] |
Cellobiose | 16.7 | 1519.1 | – | – |