A multifunctional thermophilic glycoside hydrolase from Caldicellulosiruptor owensensis with potential applications in production of biofuels and biochemicals

Table 4 CoGH1A and some reported microbial β-glucosidases with relatively high activity

Resource	GH family	Substrate	K _m (mM)	V _max (μmol mg⁻¹ min⁻¹)	k _cat (s⁻¹)	k _cat/K _m (mM⁻¹ s⁻¹)	OT(°C)	OP	References
C. owensensis	GH1	pNPGlu	1.52	4027	3750.6	2467.5	75–85	5.5	This work
C. owensensis	GH1	Cellobiose	15.65	2424	2149	137.3	75–85	5.5	This work
C. bescii	GH1	pNPGlu	3.71	–	311.6	84.0	85	6.8	[9]
C. bescii	GH1	Cellobiose	90.8	–	757.9	8.35	85	6.8	[9]
Neosartorya fischeri	GH1	pNPGlu	68	886	826.7	12.2	40	6	[46]
Pholiota adipose	GH3	pNPGlu	2.23	4390	–	–	65	5	[28]
Pholiota adipose	GH3	Cellobiose	5.60	3460	–	–	65	5	[28]
Fomitopsis pinicola	GH3	pNPGlu	1.8	1710	2990	1700	50	4.5	[47]
Thermotoga petroph	GH1	pNPGlu	2.8	42,700	87,400	30,800	80–90	7–8	[10]
Stereum hirsutum	GH1	pNPGlu	2.5	3028	–	–	65	4.5	[48]
Stereum hirsutum	GH1	Cellobiose	86	172	–	–	65	4.5	[48]
Talaromyces thermophilus	–	pNPGlu	0.25	228.7	–	–	65	5.0	[49]
Talaromyces thermophilus	–	Cellobiose	16.7	1519.1	–	–	65	5.0	[49]

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