Fig. 2

Growth of host strains (SH5, SH9, and SH9*) in glucose-containing M9 medium and mutation occurred in SH9*. Refer to Table 1 for the genotype of each strain. a Growth under aerobic condition, b growth under anaerobic condition and c difference in genome sequence between SH9 and SH9* (adapted SH9 strain). Adaptation introduced a single-point mutation only in the promoter region of pfkB, from ‘C’ to ‘T’. The mutated nucleotide is marked in red