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Table 3 Relative transcription levels of key glycolytic enzymes in SH9*_ZG after induction with different IPTG concentrations

From: Co-production of hydrogen and ethanol by pfkA-deficient Escherichia coli with activated pentose-phosphate pathway: reduction of pyruvate accumulation

Gene IPTG concentration (mM)
0 mM 0.02 mM 0.20 mM
pgi 2.3 5.2 5.3
pfkA 0.00 0.00 0.00
pfkB 63.3 58.5 40.2
gapA 4.5 17.5 34.1
edd 0.30 0.6 0.5
zwf 1.02 2222.0 7631.1
gnd 8.08 1610.4 3075.0
tktA 9.4 6.1 4.3
pflB 28.4 21.2 21.1
fhlA 0.1 0.2 0.1
udhA 0.1 0.2 0.3
adhE 2.1 4.6 35.9
pntA 4.1 ND 1.6
adhP 0.2 ND 0.5
eutG 0.0 ND 0.1
fucO 0.1 ND 0.3
yiaY 0.0 ND 0.3
yqhD 0.6 ND 1.1
yjgB 0.3 ND 0.4
mhpF 0.1 ND 0.1
aldB 0.3 ND 0.4
  1. The result was from three individual experiment repeats and the standard deviation was less than 10 %
  2. rpoD was used as the endogenous control and the transcriptional level of rpoD was considered as 1
  3. ND not determined