Table 3 Relative transcription levels of key glycolytic enzymes in SH9*_ZG after induction with different IPTG concentrations
Gene | IPTG concentration (mM) | ||
|---|---|---|---|
0 mM | 0.02 mM | 0.20 mM | |
pgi | 2.3 | 5.2 | 5.3 |
pfkA | 0.00 | 0.00 | 0.00 |
pfkB | 63.3 | 58.5 | 40.2 |
gapA | 4.5 | 17.5 | 34.1 |
edd | 0.30 | 0.6 | 0.5 |
zwf | 1.02 | 2222.0 | 7631.1 |
gnd | 8.08 | 1610.4 | 3075.0 |
tktA | 9.4 | 6.1 | 4.3 |
pflB | 28.4 | 21.2 | 21.1 |
fhlA | 0.1 | 0.2 | 0.1 |
udhA | 0.1 | 0.2 | 0.3 |
adhE | 2.1 | 4.6 | 35.9 |
pntA | 4.1 | ND | 1.6 |
adhP | 0.2 | ND | 0.5 |
eutG | 0.0 | ND | 0.1 |
fucO | 0.1 | ND | 0.3 |
yiaY | 0.0 | ND | 0.3 |
yqhD | 0.6 | ND | 1.1 |
yjgB | 0.3 | ND | 0.4 |
mhpF | 0.1 | ND | 0.1 |
aldB | 0.3 | ND | 0.4 |