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Table 2 n-Butanol titers and yields of n-butanol producing strains

From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Strain n-Butanol
Titer [mg/L] Yield [g/g glucose]
Anaerobic
 VSY10 108.08 ± 10.8 0.010 ± 0.0003
 VSY10 + 25 µM pantothenate 130.46 ± 19.7 0.012 ± 0.0008
Aerobic
 VSY10 148.21 ± 14.08 0.012 ± 0.0005
 VSY10 + 25 µM pantothenate 235.02 ± 7.74 0.013 ± 0.0005
 VSY13 165.00 ± 15.42 0.010 ± 0.0017
 VSY13 + 25 µM pantothenate 247.34 ± 8.23 0.015 ± 0.0006
 VSY15 243.20 ± 14.13 0.016 ± 0.0017
 VSY15 + pVS4ev 254.49 ± 5.89 0.017 ± 0.0001
 VSY15 + pRS62H_tdTer 448.72 ± 3.16 0.028 ± 0.0010
 VSY19 633.92 ± 19.33 0.057 ± 0.002
 VSY19 + pVS4ev 643.31 ± 17.42 0.047 ± 0.0007
 VSY19 + pRS62H_tdTer 859.05 ± 120.32 0.071 ± 0.006
  1. Titers and yields of anaerobic [1] and aerobic fermentations in SMD with or without addition of 25 µM pantothenate and with or without additional pVS4ev or pRS62H_tdTer vector are shown for strains VSY10 (Δadh16 sfa1 gpd2, with n-butanol pathway genes, coaA and adhE A267T/E568K), VSY13 (like VSY10, but adhE A267T/E568K/R577S instead of adhE A267T/E568K), VSY15 (VSY13 with pADH1_FMS1) and VSY19 (VSY15 with Δald6). The maximal titers in fermentations are shown, which is for VSY10 and VSY15/VSY19 + pRS62H_tdTer after 74 h and VSY19 strains after 50 h and for all other strains after 100 h. Yields are always calculated with values referring to samples taken after 74 or 50 h. The mean values of three independent replicates are shown with standard deviations