Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Table 2 n-Butanol titers and yields of n-butanol producing strains

Titers and yields of anaerobic [1] and aerobic fermentations in SMD with or without addition of 25 µM pantothenate and with or without additional pVS4ev or pRS62H_tdTer vector are shown for strains VSY10 (Δadh1–6 sfa1 gpd2, with n-butanol pathway genes, coaA and adhE ^A267T/E568K), VSY13 (like VSY10, but adhE ^{A267T/E568K/R577S} instead of adhE ^A267T/E568K), VSY15 (VSY13 with pADH1_FMS1) and VSY19 (VSY15 with Δald6). The maximal titers in fermentations are shown, which is for VSY10 and VSY15/VSY19 + pRS62H_tdTer after 74 h and VSY19 strains after 50 h and for all other strains after 100 h. Yields are always calculated with values referring to samples taken after 74 or 50 h. The mean values of three independent replicates are shown with standard deviations

ISSN: 2731-3654