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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression

Fig. 3

Subcellular localization and functional transport analysis of glucose transporters in Saccharomyces cerevisiae. a Glucose transporter coding sequences fused with the enhanced green fluorescence protein (eGFP) were expressed under the control of the S. cerevisiae pgk1 promoter. A clone carrying the empty vector (pRS426) was the negative control. The scale bar corresponds to 5 µm. b Complementary growth of EBY.VW4000 harboring the indicated glucose membrane carriers on maltose or glucose for 3 or 6 days, respectively. c Michaelis–Menten kinetics of radiolabeled glucose uptake by S. cerevisiae containing HGT-1, HGT-2, or GLT-1

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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