Fig. 4

Phenotypes of Δglt-1, Δ2hgt, and Δ2hgt;Δglt-1 mutants with respect to transient glucose uptake and vegetative growth on high levels of glucose. a Dynamic uptake curve of carbon-deprived mycelia of the key low- and/or high-affinity transporter-deleted strains under 1.1 mM glucose. Average values and standard deviations are shown for three biological replicates within at least two technical replicates for each sample. b Mycelial biomass and c residual glucose of the WT, Δglt-1, Δ2hgt, and Δ2hgt;Δglt-1 after cultivation for the indicated time. d Relative expression levels of hgt-1/-2 and several putative sugar transporters in the Δglt-1 mutant vs. the WT on 27.5 mM glucose. e Relative expression levels of glt-1 and several putative sugar transporters in the Δ2hgt mutant vs. the WT on 0.55 mM glucose. Data are means of triplicate; error bars indicate standard deviations