- Open Access
Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge
Biotechnology for Biofuels volume 10, Article number: 158 (2017)
Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass. Little is known about how the diverse microbial communities in anaerobic digesters (ADs) metabolize carbohydrates or which CAZymes that are present, making the ADs a unique niche to look for CAZymes that can potentiate the enzyme blends currently used in industry.
Enzymatic assays showed that functional CAZymes were secreted into the AD environments in four full-scale mesophilic Danish ADs fed with primary and surplus sludge from municipal wastewater treatment plants. Metagenomes from the ADs were mined for CAZymes with Homology to Peptide Patterns (HotPep). 19,335 CAZymes were identified of which 30% showed 50% or lower identity to known proteins demonstrating that ADs make up a promising pool for discovery of novel CAZymes. A function was assigned to 54% of all CAZymes identified by HotPep. Many different α-glucan-acting CAZymes were identified in the four metagenomes, and the most abundant family was glycoside hydrolase family 13, which contains α-glucan-acting CAZymes. Cellulytic and xylanolytic CAZymes were also abundant in the four metagenomes. The cellulytic enzymes were limited almost to endoglucanases and β-glucosidases, which reflect the large amount of partly degraded cellulose in the sludge. No dockerin domains were identified suggesting that the cellulytic enzymes in the ADs studied operate independently. Of xylanolytic CAZymes, especially xylanases and β-xylosidase, but also a battery of accessory enzymes, were present in the four ADs.
Our findings suggest that the ADs are a good place to look for novel plant biomass degrading and modifying enzymes that can potentiate biological processes and provide basis for production of a range of added-value products from biorefineries.
The use of biomass to produce more food and feed from biomass and renewable biobased chemicals, materials, and fuel requires improved carbohydrate-active enzymes (CAZymes) and/or still unknown CAZyme functions that can be added to the current enzyme cocktails used in the industry in order to optimize valorization of the biomass . CAZymes can be identified by studying how microbial communities metabolize the biomass . Unfortunately, only a fraction of the total microbial diversity can currently be cultured in the laboratory ; however, metagenomics, combined with use of synthetic genes for production of CAZymes through heterologous expression, has revolutionized our opportunities for CAZyme discovery from microbes often living in complex communities. This enables us to study microbial communities [2, 4,5,6] and also finds new enzymes for industrial uses.
Metagenomics has been used both to discover novel CAZymes and expand our understanding of the synergism between these enzymes in microbial biomass degradation. A study of switchgrass degraded in cow rumen showed that only 12% of the identified CAZymes had 75% or more identity to known proteins and 43% had less than 50% identity to known proteins. The large fraction of new enzymes demonstrates that metagenomic studies serve as an excellent starting point to discover novel CAZymes . Other metagenomics studies of microbial habitats, which differs in, e.g., temperature, pH, and oxygen availability also showed great variation in CAZymes [8,9,10,11,12]. Despite the plethora of sequencing data already analyzed, new activities within CAZyme families are continuously discovered and even new families established [13,14,15,16]. There is still much to uncover about the variety of CAZymes and their accessory proteins [1, 5]. Exploring habitats that differ significantly from already explored habitats should therefore not be neglected in pursuit of novel and more efficient CAZymes. The microorganisms in unexplored habitats may have evolved novel CAZymes in order to cope with both deconstruction of the biomass they use as carbon source and the environment their secreted enzymes must act in.
Such a unique niche is found in anaerobic digesters (ADs) fed with primary and surplus sludge from municipal wastewater treatment plants that is largely unexplored in terms of diversity of CAZymes [17,18,19,20]. ADs at municipal wastewater treatment facilities are getting more common due to a shift from only wastewater treatment to also including renewable energy production in the form of methane in the biogas produced. The ADs are complex ecosystems and the hydrolytic bacteria are extremely diverse in ADs, which give the advantage of enormous metabolic flexibility . However, little is still known about the carbohydrate metabolism in ADs and the CAZymes that are present as most metagenomic studies have focused on microorganism composition .
Here we explore four mesophilic ADs fed with surplus and surplus sludge from wastewater treatment plants for CAZymes by mining the metagenomes obtained by Illumina sequencing with Homology to Peptide Patterns (HotPep). Peptide Pattern Recognition (PPR) is a non-alignment-based approach that identifies a set of short conserved sequences, which can be used as a fingerprint when mining genomes with HotPep for CAZymes and also to predict the function of the individual enzymes .
Sample collection and DNA extraction
Biomass samples were collected from 4 full-scale Danish mesophilic AD reactors at the wastewater treatment plants Søholt, Randers, Viborg, and Frederica. All reactors have been in operation for several years and the first three receive primary sludge (precipitated wastewater) and surplus activated sludge, approx. 50% of each. In Fredericia, the feed is only surplus activated sludge and it is pretreated by thermal hydrolysis. Further details about the plant design and operation can be found elsewhere . The samples were frozen and stored until analysis. DNA was extracted from the biomass using the FastDNA Spin kit for soil (MP Biomedicals, Santa Ana, CA, USA), following the standard protocol except for four times increased bead beating duration and a sludge input volume of 50 μl .
Illumina sequencing and assembly
Illumina TruSeq DNA PCR-free libraries were prepared from DNA extracts according to the manufacturer’s protocol and paired-end sequenced on the Illumina HiSeq 2000 platform (2 × 150 bp) and Illumina MiSeq platform (v3 chemistry, 2 × 300 bp). Reads were quality-trimmed and filtered using default settings in CLC Genomics Workbench (v. 7.5.1; CLC Bio, Aarhus, Denmark). The metagenomic reads were assembled separately for each plant using default settings in CLC Genomics Workbench.
Mining and annotation
HotPep [26, 27] were used to identify CAZymes in the four metagenomes using peptide patterns generated with PPR in January 2015 for all families of glycoside hydrolases (GHs), auxiliary activities (AAs), polysaccharide lyases (PLs) and glycosyltransferases (GTs) in the CAZy database (http://www.cazy.org) . The identified CAZymes were analyzed for carbohydrate-binding domains (CBMs) and dockerin domains using dbCAN . A BlastP was performed for all identified CAZymes using the NCBI nr database (http://blast.ncbi.nlm.nih.gov/Blast.cgi) to identify the closest related known protein, and BlastP was also used for comparing the CAZymes identified in the four ADs. PhyloPhytiaS was used for taxonomical assignment of the genes coding for the identified CAZymes by searching against “Generic 2013–800 Genera” . The heatmaps were visualized with Multiexperiment Viewer software  and Circos software .
Sequence data availability
The four metagenomes were deposited in the National Center for Biotechnology Information (NCBI) and can be accessed in the Whole Genome Shotgun (WGS) under accession numbers MTKY00000000 (Søholt), MTKX00000000 (Randers), MTKZ00000000 (Viborg), and MTKW00000000 (Fredericia). See Table 1 for summary of the sequencing data.
The enzyme profile of the supernatant for the AD Søholt was investigated using 0.1% insoluble chromogenic AZurine Cross-Linked (AZCL) barley β-glucan, pachyman and curdlan, birchwood xylan, wheat arabinoxylan, and galactomannan (all Megazyme) dispersed in 1% agarose plates prepared using the buffer 0.08 M phosphoric acid, 0.08 M acetic acid, 0.08 M boric acid pH 6. The samples were sonicated and the supernatants (15 µl, undiluted) were added to triplicate wells in Petri dishes containing the different AZCL substrates and incubated at 37 °C for 3 days. Enzyme activity was confirmed by a blue halo around the sample well, indicating the presence of active enzymes that can break down the specific AZCL substrate, thereby releasing the blue, soluble dye.
Results and discussion
The anaerobic digesters
The four ADs were all typical biogas plants at municipal wastewater treatment plants. Three of them received a mixture of primary sludge (pre-settled wastewater) and surplus activated sludge (Søholt, Randers, Viborg) as feed, while Fredericia received surplus activated sludge only and it was treated by thermal hydrolysis before added to the digester. The reactors have reported loading rates of 1–2.5 kg volatile solids m−3 day−1, ammonium levels of 500–2400 mg l−1, volatile fatty acids (VFA) concentrations of 0.5–15 mmol l−1, pH of 7.1–7.8, and sludge retention times of 15–35 days. A recent survey of the microbial community composition in Danish ADs showed that the first three reactors had a very similar composition and it was typical for Danish digesters . Fredericia had a slightly different community composition (unpublished results). Primary sludge is the particulate fraction of wastewater, consisting of approx. 30% lipids, carbohydrates, and proteins . Surplus sludge is the same as activated sludge and it consists primarily of microorganisms .
Secretion of CAZymes in the anaerobic digesters
As preliminary feasibility study, the supernatant of the AD Søholt sampling was tested for CAZyme activities. Activity against birchwood and wheat arabinoxylan was detected (Fig. 1) showing that xylanolytic enzymes were secreted by the microorganisms. Activity against barley β-glucan, pachyman, and curdlan (data not shown) suggests the presences of β-glucanolytic CAZymes and possibly also cellulytic CAZymes in the supernatant. No activity was detected against galactomannan (Fig. 1). The results indicate that functional CAZymes were secreted into the AD environments.
Identified carbohydrate-active enzymes
To explore the obtained metagenomes for CAZymes, HotPep [26, 27] was used to mine the four metagenomes for CAZymes. In total 19,335 catalytic domains were identified of which 10,374 were GHs, 6336 were GTs, 2064 were CEs, and 202 were PLs (Table 2). The numbers for the individual ADs are listed in Table 2. 98 of 133 GH, 15 of 17 CE, 14 of 25 PL, and 41 of 99 GT families were represented in the metagenomes (Fig. 2a–d). The diverse repertoire of CAZyme genes (Fig. 3a–d) provides the basis for a flexible carbohydrate metabolism within the microbial community and for discovery of novel CAZymes. A small number (718) of auxiliary activity CAZymes were also identified (Additional file 1); however, these oxidative enzymes have very limited access to oxygen in the ADs. Hence, they are not relevant for the degradation in the anaerobic digesters.
The CAZymes identified in AD Randers showed low homology to the CAZymes identified in AD Fredericia and AD Søholt; however, high homology to the ones identified in AD Viborg (Table 3; Additional file 2). The CAZymes identified in AD Viborg showed modest homology to the CAZymes identified in AD Søholt and low homology to the CAZymes identified in AD Fredericia, which was also the case when comparing ADs Søholt and Fredericia (Table 3; Additional file 2).
The diversity of CAZyme families has been explored in several anaerobic habitats such as cow rumen [7, 34, 35], muskoxen rumen , yak rumen , termite gut [38,39,40,41], wallaby gut , giant panda gut , elephant faces , and fresh fecal samples from Yunnan snub-nosed monkey . The microbial communities in the four ADs investigated had a higher diversity in identified CAZyme families than found in the above-mentioned rumen and gut samples. This suggests that the microbial community in the ADs (including the microbes coming from the feed to the four ADs) encounters a much more diverse carbohydrate composition than those in the above-mentioned habitats. This shows that the ADs studied is a great place to look for novel microbial enzymes to potentiate the enzyme cocktails used to day for biomass degradation.
Unfortunately, in most of the above-mentioned studies, a specific function is not assigned to the identified CAZymes, and since many CAZyme families include enzymes with different functions, a comparison at family level does not make sense in terms of what the microorganisms found in the ecological niche can degrade. HotPep is trained to assign a function to CAZymes if an activity for a group created by PPR has been determined according to the CAZy database . HotPep assigned a function to 54% of the 19,335 identified CAZymes in the four ADs (Table 2), showing that our knowledge of a lot of the CAZy subfamilies lack behind as HotPep only assigns a function to a PPR group if a member of the PPR group has been characterized [26, 27]. It is reasonable to assume that new specificities could be identified by characterizing CAZymes from these PPR groups.
The four ADs were very similar in terms of percentage of CAZymes with an assigned function (Søholt 56%, Randers 52%, Viborg 54%, and Frederica 55%) (Table 2), suggesting that ADs at municipal wastewater treatment plants in general is a highly promising place to look for novel CAZymes. Looking at the different types of CAZymes, the picture differs as a function has been assigned to 64% GHs, 48% GTs, 40% CEs, 36% PLs, and 25% AAs (Table 2), which reflects the number of characterized members of the different CAZymes.
Low homology to known proteins suggests that the CAZymes could be optimized for specific functions, which could be exploited in industrial process . 30% of the identified CAZymes in the four metagenomes showed 50% or lower identity to known proteins and less than 3% showed 95% or higher identity to known proteins (Additional file 1). This suggests that the ADs at municipal wastewater treatment plants provide a unique and unexploited ecological niche for discovery of CAZymes with novel biochemical and physicochemical properties.
Microbial origin of the identified carbohydrate-active enzymes
A meta study showed that Chloroflexi, Proteobacteria, Firmicutes, and Bacteroidetes are the dominating phyla in ADs . However, large variations are found within the individual ADs due to differences in the living conditions for the microbes, reactor type, and the biomass the AD are fed with [18, 46,47,48,49,50,51,52,53,54,55]. A recent survey of Danish ADs showed that besides the above-mentioned phyla, the 25 most abundant bacterial species are of Actinobacteria and Spirochaetae .
We used PhylopythiaS  to investigate which phyla harbored the identified CAZymes from the four ADs. A major part, 38%, of the CAZymes were not assigned to a phylum due to the lack of reference genomes (Fig. 4). Proteobacteria, Firmicutes, Acidobacteria, Actinobacteria, and Bacteroidetes accounted for 55% of the identified CAZymes, while up to 21 other phyla accounted for the remaining CAZymes (7%) (Fig. 4; Additional file 3).
The diverse repertoire and distinct distribution of CAZymes on the bacterial taxonomic groupings within the four ADs suggest that the microbial community within the four ADs is likely to experience changes in the carbohydrate content, compared to, e.g., the uniform diet of ruminants. Hence, this makes the AD a unique place to look for a diverse type of CAZymes.
Abundance, variety, and taxonomic distribution of α-glucan, cellulose, and xylan hydrolyzing CAZymes
We looked into the five dominating phyla; Proteobacteria, Firmicutes, Bacteroidetes, Acidobacteria, and Actinobacteria. These five constitute the core of the origin of the CAZymes identified in the four ADs (Fig. 4 and Additional file 3), and in order to get a better understanding of which of these phyla it would be promising to look into for discovery of novel α-glucans-, xylans-, and cellulose-acting CAZymes.
ADs fed with primary sludge from wastewater treatment plants contain large amounts of cellulose, which stems mainly from toilet paper and constitutes about 35% of the suspended solids in the influent . This is reflected in the vast number of cellulytic enzymes identified in all four ADs (Fig. 3a). The cellulose found in toilet paper is highly accessible to hydrolytic enzymes due to the extensive chemical and heat treatments during production [57, 58] and may therefore be less recalcitrant than when it originates directly from the natural source. It is therefore not surprising that the cellulytic enzymes identified in the four ADs studied here were limited almost exclusively to endoglucanases (EC 220.127.116.11) that randomly hydrolyze the β-1,4-linkages in amorphous regions of cellulose , and β-glucosidases (18.104.22.168) (Fig. 3a) that hydrolyze the same linkage in cellobiose and cello oligosaccharides thereby producing glucose . The endoglucanases identified in the four ADs are mainly from GH5 and GH9 (Additional file 4), similar to what is seen in other anaerobic habitats [17, 42, 60]. A few endoglucanases belong to GH6, GH8, GH44, GH45, and GH51 (Additional file 4). The β-glucosidases are mainly from GH1 and GH3 (Additional file 4), which is also the case in other anaerobic environments [17, 42, 60], and a single gene stems from GH116 (Additional file 4). Cellulytic enzymes from bacteria are sometimes found in cellulosomes, which are enzyme complexes consisting of several cellulytic enzymes and other proteins involved in cellulose degradation . However, no dockerin domains were identified in the cellulytic enzymes identified in the four ADs (Additional file 5) suggesting that the cellulases in the ADs studied operates independently.
Many CAZymes secreted into the sludge by the microorganisms are trapped in the exopolysaccharide matrix synthesized by the microorganisms , which likely ensures that the CAZymes remain close to the cell, hence also the produced glucose, which also limits the usefulness of cellulosomes. Additionally, CBMs from CBM3, CBM4, CBM17, CBM28, CBM30, and CBM46 known to bind to non-crystalline cellulose were appended to some of the cellulytic enzymes (Fig. 5; Additional file 4).
The majority of the β-glucosidases and endoglucanases are from Proteobacteria (Fig. 6). Interestingly, most Proteobacteria are opportunist, which means that they do not harbor genes encoding for both β-glucosidases and endoglucanases , and when searching for novel independently operating β-glucosidases and endoglucanases in the four metagenomes, the genes originating from Proteobacteria will be a good starting point.
Xylans are often more complex than cellulose in the sense that the β-1,4-xylose backbone is often decorated with, e.g., single and/or double substituted α-1,2- and/or α-1,3-arabinofuranose that can be decorated with ferulic acid. 4-O-methyl-d-glucuronic acid linked α-1,2 to the xylose is another common substitution and so is single and/or double substituted acetyl linked O-2, O-3 and/or O-4 to the xylose. The position, type, and amount of substitutions vary greatly with the origin of the xylan [63,64,65,66]. Hence, many different CAZymes are needed for complete saccharification of xylans [65,66,67,68], and several of these are present in the four ADs in great numbers. Whether xylan is present or not in the sludge fed into the four ADs is unknown. However, xylan could be present in the vast amount of degrading toilet paper present in the municipal wastewater [57, 58].
Xylanolytic enzymes are present in great numbers in the four metagenomes, especially xylanases (EC 22.214.171.124) and β-xylosidase (EC 126.96.36.199). Both of these hydrolyze 1,4-β-d linkages between the xylose residues constitute the main chain of xylan . The majority of the xylanases are of GH10; however, some are of GH8 (Additional file 4). The substrate specificity of GH10 xylanases is heteroxylans with a high degree of substitution, while GH8 xylanases have a more diverse substrate specificity, but with limited activity towards highly substituted xylans , suggesting that the microorganisms encounters both types of xylans. The less substituted xylans may also be a product of the xylanolytic accessory enzymes mentioned below, which then allows the GH8 to degrade the xylan main chain. β-xylosidase hydrolyzes the oligosaccharides produced by the xylanases into xylose or smaller oligosaccharides , and in general these are classified as GH3, GH39, GH43, and GH120 members for all the ADs (Additional file 4).
Xylanolytic accessory enzymes like α-l-arabinofuranosidases (EC 188.8.131.52), α-glucuronidases (EC 184.108.40.206), acetyl xylan esterases (AXEs) (EC 220.127.116.11), and ferulic acid esterases (FAEs) (EC 18.104.22.168), which are important for degradation of xylan from various sources , are all present in the four metagenomes (Fig. 3a–b).
Among the many FAEs identified in the four ADs, there are several FAEs linked to a CBM48 (Additional file 5), which are known to bind α-glucans like starch  that to the best of our knowledge does not contain ferulic acid. One could speculate that some of the bacteria in the ADs produce α-glucan exopolysaccharides (EPS) that are ferulated, which to the best of our knowledge have not been shown to exist.
Several xylanolytic CAZymes consisting of two or more catalytic domains are present in the four metagenomes (Additional file 5), which ensure that the functions required for degrading highly substituted xylans are in close proximity when secreted into the EPS matrix. Additionally, CBMs are in some cases appended to both these multidomain xylanolytic enzymes and individual domains (Additional file 5).
Proteobacteria is the dominating phylum when it comes to xylanolytic CAZymes, although it accounts for a smaller relative abundance of the xylanolytic than of the cellulytic CAZymes (Fig. 7). Acidobacteria has all the required CAZymes to degrade arabinoglucuronoxylan except endo-1,4-β-xylanases (Fig. 7), which are essential for xylan degradation.
Glucan active enzymes
GH13 is the most abundant GH family in the four metagenomes (Fig. 2a; Additional file 1). GH13 is a family of α-glucan-acting CAZymes  suggesting that the microbial communities encounter α-glucans (e.g., starch and glycogen). Glycogen-accumulating bacteria are abundant in ADs [72, 73] and GH13 CAZymes are necessary for hydrolysis of these polysaccharides when the glucose is needed for energy [16, 74]. However, this alone may not explain the large number of α-glucan-degrading CAZymes (Fig. 3a) and many of the genes encoding for GH13 enzymes are not of glycogen-accumulating bacteria. Further, many of the GH13 enzymes are extracellular (data not shown). Some bacteria produce α-glucan EPSs though [75, 76], which other bacteria could utilize as a carbon source if their α-glucan-acting CAZymes can hydrolyze the EPSs. Very complex EPS could potentially be present in the four ADs. Complete degradation of these would therefore require a large number of different CAZymes, which can also be found in the metagenomes from the four ADs (Fig. 3a). These EPSs may differ significantly from known polysaccharides, which may have resulted in evolution of novel functions of α-glucan-acting CAZymes in order to enable the bacteria to degrade the EPSs.
Species of Proteobacteria and Firmicutes are represented in all the α-glucan-degrading CAZymes functions identified in this study (Fig. 8). Bacteroidetes, Actinobacteria, and Acidobacteria are not represented in all the α-glucan-degrading CAZymes functions (Fig. 8). However, it is difficult to predict in which phyla superior α-glucan-acting CAZymes can be found due to our lack of understanding of their presumed substrate.
We identified nearly 20,000 CAZymes in the four ADs studied here and 30% of them showed 50% or lower identity to known proteins. This suggests that ADs fed with primary sludge and surplus sludge from municipal wastewater treatment plants are in fact a unique place to look for novel CAZymes. Further, we were only able to assign a function to 54% of the CAZymes by our unique bioinformatics approach, which demonstrates that there is still much to uncover about the functionality of CAZymes as HotPep only assigns a function to a PPR group if a CAZyme within the group have already been characterized. This suggests that the ADs are a good place to look for novel plant biomass degrading and modifying enzymes that can potentiate biological processes and provide basis for production of a range of added-value products from biorefineries.
Harris PV, Xu F, Kreel NE, Kang C, Fukuyama S. New enzyme insights drive advances in commercial ethanol production. Curr Opin Chem Biol. 2014;19:162–70.
Rosnow JJ, Anderson LN, Nair RN, Baker ES, Wright AT. Profiling microbial lignocellulose degradation and utilization by emergent omics technologies. Crit Rev Biotechnol. 2017. doi:10.1080/07388551.2016.1209158.
Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol. 1990;56:1919–25.
Montella S, Amore A, Faraco V, Montella S, Amore A, Faraco V. Metagenomics for the development of new biocatalysts to advance lignocellulose saccharification for bioeconomic development. Crit Rev Biotechnol. 2016;36:998–1009.
Cragg SM, Beckham GT, Bruce NC, Bugg TD, Distel DL, Dupree P, et al. Lignocellulose degradation mechanisms across the tree of life. Curr Opin Chem Biol. 2015;29:108–19.
Morrison M, Pope PB, Denman SE, McSweeney CS. Plant biomass degradation by gut microbiomes: more of the same or something new? Curr Opin Biotechnol. 2009;20:358–63.
Hess M, Sczyrba A, Egan R, Kim T-W, Chokhawala H, Schroth G, et al. Metagenomic discovery of biomass-degrading genes and genomes from cow rumen. Science. 2011;331:463–7.
Mhuantong W, Charoensawan V, Kanokratana P, Tangphatsornruang S, Champreda V. Comparative analysis of sugarcane bagasse metagenome reveals unique and conserved biomass-degrading enzymes among lignocellulolytic microbial communities. Biotechnol Biofuels. 2015;8:16.
Vester JK, Glaring MA, Stougaard P. Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing. Microb Cell Fact. 2014;13:72.
D’haeseleer P, Gladden JM, Allgaier M, Chain PSG, Tringe SG, Malfatti SA, et al. Proteogenomic analysis of a thermophilic bacterial consortium adapted to deconstruct switchgrass. PLoS ONE. 2013;8:e68465.
Allgaier M, Reddy A, Park JI, Ivanova N, D’haeseleer P, Lowry S, et al. Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community. PLoS ONE. 2010;5:e8812.
Ilmberger N, Güllert S, Dannenberg J, Rabausch U, Torres J, Wemheuer B, et al. A Comparative metagenome survey of the fecal microbiota of a breast- and a plant-fed Asian elephant reveals an unexpectedly high diversity of glycoside hydrolase family enzymes. PLoS ONE. 2014;9:e106707.
Shimizu M, Kaneko Y, Ishihara S, Mochizuki M, Sakai K, Yamada M, et al. Novel β-1,4-mannanase belonging to a new glycoside hydrolase family in Aspergillus nidulans. J Biol Chem. 2015;290:27914–27.
Rigden DJ, Eberhardt RY, Gilbert HJ, Xu Q, Chang Y, Godzik A. Structure- and context-based analysis of the GxGYxYP family reveals a new putative class of glycoside hydrolase. BMC Bioinform. 2014;15:196.
Vuillemin M, Claverie M, Brison Y, Séverac E, Bondy P. Characterization of the first α-(1→3) branching sucrases of GH70 family. J Biol Chem. 2016;291:7687–702.
Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res. 2014;42:D490–5.
Wei Y, Zhou H, Zhang J, Zhang L, Geng A, Liu F, et al. Insight into dominant cellulolytic bacteria from two biogas digesters and their glycoside hydrolase genes. PLoS ONE. 2015;10:e0129921.
Schlüter A, Bekel T, Diaz NN, Dondrup M, Eichenlaub R, Gartemann KH, et al. The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology. J Biotechnol. 2008;136:77–90.
Xia Y, Ju F, Fang HHP, Zhang T. Mining of novel thermo-stable cellulolytic genes from a thermophilic cellulose-degrading consortium by metagenomics. PLoS ONE. 2013;8:e53779.
Yan X, Geng A, Zhang J, Wei Y, Zhang L, Qian C, et al. Discovery of (hemi-) cellulase genes in a metagenomic library from a biogas digester using 454 pyrosequencing. Appl Microbiol Biotechnol. 2013;97:8173–82.
Alvarado A, Montañez-Hernández LE, Palacio-Molina SL, Oropeza-Navarro R, Luévanos-Escareño MP, Balagurusamy N. Microbial trophic interactions and mcrA gene expression in monitoring of anaerobic digesters. Front Microbiol. 2014;5:597.
Shinkai T, Mitsumori M, Sofyan A, Kanamori H, Sasaki H, Katayose Y, Takenaka A. Comprehensive detection of bacterial carbohydrate-active enzyme coding genes expressed in cow rumen. Anim Sci J. 2016;87:1363–70.
Busk PK, Lange L. Function-based classification of carbohydrate-active enzymes by recognition of short, conserved peptide motifs. Appl Environ Microbiol. 2013;79:3380–91.
Kirkegaard RH, Mcilroy SJ, Kristensen JM, Nierychlo M, Karst SM, Dueholm MS, et al. Identifying the abundant and active microorganisms common to full-scale anaerobic digesters. bioRxiv. 2016. doi:10.1101/104620.
Albertsen M, Karst SM, Ziegler AS, Kirkegaard RH, Nielsen PH. Back to basics—the influence of DNA extraction and primer choice on phylogenetic analysis of activated sludge communities. PLoS ONE. 2015;10:e0132783.
Busk PK, Lange L. A novel method of providing a library of n-mers or biopolymers. 2012. EP11152232.2.
Busk PK, Lange M, Pilgaard B, Lange L. Several genes encoding enzymes with the same activity are necessary for aerobic fungal degradation of cellulose in nature. PLoS ONE. 2014;9:e114138.
Yin Y, Mao X, Yang J, Chen X, Mao F, Xu Y. DbCAN: a web resource for automated carbohydrate-active enzyme annotation. Nucleic Acids Res. 2012;40:445–51.
Patil KR, Roune L, McHardy AC. The phyloPythiaS web server for taxonomic assignment of metagenome sequences. PLoS ONE. 2012;7:e38581.
Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, et al. TM4 microarray software suite. Methods Enzymol. 2006;411:134–93.
Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, et al. Circos: an information aesthetic for comparative genomics. Genome Res. 2009;19:1639–45.
Kirkegaard RH, Dueholm MS, Mcilroy SJ, Nierychlo M, Karst SM, Albertsen M, et al. Genomic insights into members of the candidate phylum Hyd24-12 common in mesophilic anaerobic digesters. ISME J. 2016;10:1–13.
Raunkjær E, Hvitved-Jacobsen T, Nielsen PH. Measurements of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res. 1994;28:251–62.
Dai X, Tian Y, Li J, Su X, Wang X, Zhao S, et al. Metatranscriptomic analyses of plant cell wall polysaccharide degradation by microorganisms in cow rumen. Appl Environ Microbiol. 2015;81:1375–86.
Brulc JM, Antonopoulos DA, Miller MEB, Wilson MK, Yannarell AC, Dinsdale EA, et al. Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. PNAS. 2009;106:1948–53.
Qi M, Wang P, O’Toole N, Barboza PS, Ungerfeld E, Leigh MB, et al. Snapshot of the eukaryotic gene expression in muskoxen rumen—a metatranscriptomic approach. PLoS ONE. 2011;6:e20521.
Dai X, Zhu Y, Luo Y, Song L, Liu D, Liu L, et al. Metagenomic insights into the fibrolytic microbiome in yak rumen. PLoS ONE. 2012;7:e40430.
Do TH, Nguyen TT, Nguyen TN, Le QG, Nguyen C, Kimura K, et al. Mining biomass-degrading genes through Illumina-based de novo sequencing and metagenomic analysis of free-living bacteria in the gut of the lower termite Coptotermes gestroi harvested in Vietnam. J Biosci Bioeng. 2014;118:665–71.
Bastien G, Arnal G, Bozonnet S, Laguerre S, Ferreira F, Fauré R, et al. Mining for hemicellulases in the fungus-growing termite Pseudacanthotermes militaris using functional metagenomics. Biotechnol Biofuels. 2013;6:78.
Warnecke F, Luginbühl P, Ivanova N, Ghassemian M, Richardson TH, Stege JT, et al. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature. 2007;450:560–5.
Saadeddin A. The complexities of hydrolytic enzymes from the termite digestive system. Crit Rev Biotechnol. 2014;34:115–22.
Pope PB, Denman SE, Jones M, Tringe SG, Barry K, Malfatti SA, et al. Adaptation to herbivory by the Tammar wallaby includes bacterial and glycoside hydrolase profiles different from other herbivores. PNAS. 2010;107:14793–8.
Zhu L, Wu Q, Dai J, Zhang S, Wei F. Evidence of cellulose metabolism by the giant panda gut microbiome. PNAS. 2011;108:17714–9.
Xu B, Xu W, Li J, Dai L, Xiong C, Tang X, et al. Metagenomic analysis of the Rhinopithecus bieti fecal microbiome reveals a broad diversity of bacterial and glycoside hydrolase profiles related to lignocellulose degradation. BMC Genom. 2015;16:174.
Nelson MC, Morrison M, Yu Z. A meta-analysis of the microbial diversity observed in anaerobic digesters. Bioresour Technol. 2011;102:3730–9.
Abendroth C, Vilanova C, Günther T, Luschnig O, Porcar M. Eubacteria and archaea communities in seven mesophile anaerobic digester plants in Germany. Biotechnol Biofuels. 2015;8:87.
Wirth R, Kovács E, Maróti G, Bagi Z, Rákhely G, Kovács KL. Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing. Biotechnol Biofuels. 2012;5:41.
Krause L, Diaz NN, Edwards RA, Gartemann KH, Krömeke H, Neuweger H, et al. Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. J Biotechnol. 2008;136:91–101.
Jaenicke S, Ander C, Bekel T, Bisdorf R, Dröge M, Gartemann KH, et al. Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing. PLoS ONE. 2011;6:e14519.
Liu A-C, Chou C-Y, Chen L-L, Kuo C-H. Bacterial community dynamics in a swine wastewater anaerobic reactor revealed by 16S rDNA sequence analysis. J Biotechnol. 2015;194:124–31.
Klocke M, Mähnert P, Mundt K, Souidi K, Linke B. Microbial community analysis of a biogas-producing completely stirred tank reactor fed continuously with fodder beet silage as mono-substrate. Syst Appl Microbiol. 2007;30:139–51.
Xia Y, Chin FYL, Chao Y, Zhang T. Phylogeny-structured carbohydrate metabolism across microbiomes collected from different units in wastewater treatment process. Biotechnol Biofuels. 2015;8:172.
Campanaro S, Treu L, Kougias PG, De Francisci D, Valle G, Angelidaki I. Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy. Biotechnol Biofuels. 2016;9:26.
Solli L, Håvelsrud OE, Horn SJ, Rike AG. A metagenomic study of the microbial communities in four parallel biogas reactors. Biotechnol Biofuels. 2014;7:146.
Duda RM, da Silva Vantini J, Martins LS, de Mello Varani A, Lemos MVF, Ferro MIT, et al. A balanced microbiota efficiently produces methane in a novel high-rate horizontal anaerobic reactor for the treatment of swine wastewater. Bioresour Technol. 2015;197:152–60.
Ruiken CJ, Breuer G, Klaversma E, Santiago T, van Loosdrecht MCM. Sieving wastewater—cellulose recovery, economic and energy evaluation. Water Res. 2013;47:43–8.
Lee SH, Lee HL, Youn HJ. Adsorption of xylan onto cellulose fibers pretreated with cationic polyelectrolyte and its effect on paper properties. BioResources. 2015;10:851–65.
Deutschle AL, Römhild K, Meister F, Janzon R, Riegert C, Saake B. Effects of cationic xylan from annual plants on the mechanical properties of paper. Carbohydr Polym. 2014;102:627–35.
Jordan DB, Bowman MJ, Braker JD, Dien BS, Hector RE, Lee CC, et al. Plant cell walls to ethanol. Biochem J. 2012;442:241–52.
Pope PB, Mackenzie AK, Gregor I, Smith W, Sundset MA, McHardy AC, et al. Metagenomics of the svalbard reindeer rumen microbiome reveals abundance of polysaccharide utilization loci. PLoS ONE. 2012;7:e38571.
Frolund B, Griebe T, Nielsen P. Enzymatic activity in the activated sludge flocmatrix. Appl Microbiol Biotechnol. 1995;43:755–61.
Berlemont R, Martiny AC. Phylogenetic distribution of potential cellulases in bacteria. Appl Environ Microbiol. 2013;79:1545–54.
Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;61:263–89.
Rennie EA, Scheller HV. Xylan biosynthesis. Curr Opin Biotechnol. 2014;26:100–7.
Busse-Wicher M, Grantham NJ, Lyczakowski JJ, Nikolovski N, Dupree P. Xylan decoration patterns and the plant secondary cell wall molecular architecture. Biochem Soc Trans. 2016;44:74–8.
Lagaert S, Pollet A, Courtin CM, Volckaert G. β-xylosidases and α-l-arabinofuranosidases: accessory enzymes for arabinoxylan degradation. Biotechnol Adv. 2014;32:316–32.
Topakas E, Christakopoulos P. Microbial xylanolytic carbohydrate. In: Polaina J, MacCabe AP, editors. Industrial enzymes. Dordrecht: Springer; 2007. p. 83–97.
Biely P, Singh S, Puchart V. Towards enzymatic breakdown of complex plant xylan structures: State of the art. Biotechnol Adv. 2016;34:1260–74.
Berrin J-G, Juge N. Factors affecting xylanase functionality in the degradation of arabinoxylans. Biotechnol Lett. 2008;30:1139–50.
Pollet A, Delcour JA, Courtin CM. Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families. Crit Rev Biotechnol. 2010;30:176–91.
Janeček Š, Svensson B, MacGregor EA. Structural and evolutionary aspects of two families of non-catalytic domains present in starch and glycogen binding proteins from microbes, plants and animals. Enzyme Microb Technol. 2011;49:429–40.
Albertsen M, McIlroy SJ, Stokholm-Bjerregaard M, Karst SM, Nielsen PH. “Candidatus Propionivibrio aalborgensis”: a novel glycogen accumulating organism abundant in full-scale enhanced biological phosphorus removal plants. Front Microbiol. 2016;7:1–17.
Mcilroy SJ, Albertsen M, Andresen EK, Saunders AM, Kristiansen R, Stokholm-Bjerregaard M, et al. “Candidatus Competibacter”-lineage genomes retrieved from metagenomes reveal functional metabolic diversity. ISME J. 2013;8:613–24.
Smith AM, Zeeman SC, Smith SM. Starch degradation. Annu Rev Plant Biol. 2005;56:73–98.
van Hijum SA, Kralj S, Ozimek LK, Dijkhuizen L, van Geel-schutten IGH. Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria. Microbiol Mol Biol Rev. 2006;70:157–76.
Leemhuis H, Pijning T, Dobruchowska JM, van Leeuwen SS, Kralj S, Dijkstra BW, et al. Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications. J Biotechnol. 2013;163:250–72.
LL, PKB, PHN, KLN, and CW conceived the study; PHN obtained the samples and metagenomes; WJZ and LL planed and performed the ACZL enzymatic assays; PKB generated the PPR grouping of the CAZymes; PKB, BP, and CW mined the metagenomes with PPR; BP and CW performed the taxonomic assignment and the search for CBMs, dockerin domains and multidomain CAZymes; CW and LL analyzed the data and wrote the paper with inputs from all co-authors. All authors read and approved the final manuscript.
LL, PKB, BP, and CW initiated the study while affiliated to Aalborg University prior to the transfer to Technical University of Denmark, and we would like to thank Aalborg University for the opportunity carry out some of the study there. Rasmus Kirkegaard is thanked for sequencing the metagenomes.
The authors declare that they have no competing interests.
Availability of data and materials
The four metagenomes were deposited in the National Center for Biotechnology Information (NCBI) and can be accessed in the Whole Genome Shotgun (WGS) under Accession Numbers MTKY00000000 (Søholt), MTKX00000000 (Randers), MTKZ00000000 (Viborg) and MTKW00000000 (Fredericia). See Table 1 for summary of the sequencing data.
The work was supported by the Villum Foundation (Grant Number VKR022796). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Wilkens, C., Busk, P.K., Pilgaard, B. et al. Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge. Biotechnol Biofuels 10, 158 (2017). https://doi.org/10.1186/s13068-017-0840-y
- Carbohydrate-active enzymes
- Anaerobic digester
- Wastewater treatment
- Glycoside hydrolase
- Carbohydrate esterase
- Polysaccharide lyase
- Carbohydrate-binding module
- Enzyme discovery
- Peptide pattern recognition
- Carbohydrate hydrolysis