Open Access

Erratum to: Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system

Biotechnology for Biofuels201710:155

https://doi.org/10.1186/s13068-017-0843-8

Received: 6 June 2017

Accepted: 8 June 2017

Published: 19 June 2017

The original article was published in Biotechnology for Biofuels 2016 9:39

Erratum to: Biotechnol Biofuels (2016) 9:39 DOI 10.1186/s13068-016-0452-y

After the publication of the article [1], it was brought to our attention that some of the data in Table 2 were incorrect. Please find a correct and updated version of Table 2 in the erratum. Following this Fig. 1 has also been updated; the correct version of Fig. 1 is given in this erratum.
Table 2

Key process parameters of anaerobic glucose conversion of P. putida F1 in the anode compartment of a BES using [Co(bipy)3]3+/2+ or [Fe(CN)6]3−/4− as electron acceptors while poising the anode at +0.697 V vs SHE

 

[Co(bipy)3]3+/2+

[Fe(CN)6]3−/4−

Carbon balance (%)

99.6

97.6

Coulombic efficiency (%)

98.5

93.3

Yields (molproduct/molglucose)

 Y 2KGA

0.90 ± 0.03

0.90 ± 0.02

 Y acetic acid

0.073 ± 0.008

0.144 ± 0.012

 Y gluconic acid

0.31 ± 0.06

0.09 ± 0.03

 

0.25 ± 0.03

0.09 ± 0.04

 Y electrons

3.94 ± 0.11

3.88 ± 0.07

Rates (mmol/(gCDW h))

 r glucose

−0.26 ± 0.04

−0.35 ± 0.07

 r acetic acid

0.019 ± 0.003

0.051 ± 0.010

 r 2KGA

0.23 ± 0.04

0.32 ± 0.06

 r gluconic acid

0.08 ± 0.02

0.03 ± 0.01

 

−0.06 ± 0.01

−0.03 ± 0.02

 r electrons

1.02 ± 0.18

1.37 ± 0.26

Data are fitted with linear regression using datasets from ten ([Fe(CN)6]3−/4−) and four ([Co(bipy)3]3+/2+) biological replicates with a total of 79 and 36 samples, respectively (compare Additional file 1: Fig. S3). Carbon balance is calculated from the fitted rates considering carbon content of molecules and assuming equimolar CO2 production when making acetate from glucose. Gluconic acid is a product in the first 100 h and serves as a substrate thereafter, hence 2 yields and rates are given

Fig. 1

Change of biomass (triangles, a), pH (squares, b) and electron production (circles, b) in the anode compartment of a BES reactor of P. putida F1 with K3[Fe(CN)6] as electron acceptor in control (black symbols) and closed circuit with the anode potential poised at +0.697 V (white symbols). Data have been averaged from ten (closed circuit) and three (control) biological replicates with a total of 79 and 30 samples, respectively. Means and standard deviations (X and Y error bars) are given [average sample size n = 7 (closed circuit); exact sample size n = 3 (control)]

Also, during the calculation of specific glucose uptake rate, the authors mistakenly used the unit mmol/L as mmol, and therefore it caused some errors in the calculations of production rate (Table 2) and ATP regeneration rate (Section “Flux balance analysis”—[1]) which need to be corrected. The corrected ATP regeneration rates are 0.02 and 0.38 mmolATP/(gCDW h) for [Co(bpy)3](ClO4)2 from glucose oxidation and membrane-bound ATP synthase respectively, while those numbers for K3[Fe(CN)6] are 0.05 and 0.64 mmolATP/(gCDW h), respectively.

Notes

Declarations

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Authors’ Affiliations

(1)
Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland
(2)
Advanced Water Management Centre (AWMC), The University of Queensland
(3)
School of Chemistry and Molecular Biosciences, The University of Queensland
(4)
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University

Reference

  1. Lai B, Yu S, Bernhardt PV, Rabaey K, Virdis B, Krömer JO. Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system. Biotechnol Biofuels. 2016;9:39. doi:https://doi.org/10.1186/s13068-016-0452-y.View ArticleGoogle Scholar

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© The Author(s) 2017

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