Synthetic CO2-fixation enzyme cascades immobilized on self-assembled nanostructures that enhance CO2/O2 selectivity of RubisCO

Table 2 Activities measured for the conversion of glucose to 3-PGA in nanostructure-multienzyme complexes with R. rubrum form II (FII) RubisCO

Enzyme cascade	Combined activity^a (μmoles/min-mg)	% Activity ret.^b
Unbound FII and glucose pathway^c	0.259	100
FII and glucose pathway^c in nanotube A	0.100	39
FII and glucose pathway^c in nanotube B	0.110	42
FII and glucose pathway^c in nanofiber C	0.115	44

^aCalculated from stable ¹⁴CO₂ fixed
^bActivities retained are presented relative to an unbound enzyme mixture comprising all enzymes. Activity numbers are from one of the two assays conducted with independently prepared and assayed nanostructure complexes, which gave similar results
^cGlucose pathway comprises all enzymes that constitute pathway 2

ISSN: 2731-3654