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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

Fig. 5

TLC analysis of neutral lipids synthesized in recombinant yeast mutant H1246. a WE synthesis in recombinant yeast H1246 expressing TrWSD4. WEs and TAG are shown as reference lipid standards. 13, lipid samples extracted from three independent yeast transformants H1246:pESC-URA fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol; 46, lipid samples extracted from three independent yeast transformants H1246:TrWSD4 fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol. b WE synthesis in recombinant yeast H1246 expressing TrWSD5. WEs and TAG are shown as reference lipid standards. 13, lipid samples extracted from three independent yeast transformants H1246:pESC-URA fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol; 46, lipid samples extracted from three independent yeast transformants H1246:TrWSD5 fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol

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