Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13068-017-0869-y/MediaObjects/13068_2017_869_Fig5_HTML.gif)
TLC analysis of neutral lipids synthesized in recombinant yeast mutant H1246. a WE synthesis in recombinant yeast H1246 expressing TrWSD4. WEs and TAG are shown as reference lipid standards. 1–3, lipid samples extracted from three independent yeast transformants H1246:pESC-URA fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol; 4–6, lipid samples extracted from three independent yeast transformants H1246:TrWSD4 fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol. b WE synthesis in recombinant yeast H1246 expressing TrWSD5. WEs and TAG are shown as reference lipid standards. 1–3, lipid samples extracted from three independent yeast transformants H1246:pESC-URA fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol; 4–6, lipid samples extracted from three independent yeast transformants H1246:TrWSD5 fed with 0.1% (w/v) palmitic acid together with 0.1% (w/v) hexadecanol